Study of the alkyl chain length on laccase stability and enzymatic kinetic with imidazolium ionic liquids

• Tipo: Artigo em Revista Científica Internacional
• Data: 2011

Autores

Oscar Rodríguez (Autor) FEUP; Raquel O. Cristóvão (Autor) FEUP; Ana P. M. Tavares (Autor) FEUP; Eugénia A. Macedo (Autor) FEUP

Revista

A Revista está pendente de validação pelos Serviços Administrativos.

Título: Appl Biochem Biotechnol - Applied Biochemistry and Biotechnology

Vol. 164 Nº 4

Páginas: 524-533

ISSN: 0273-2289

Indexação

ISI Web of Knowledge - 36 Citações

ISI Web of Science

Scopus - 39 Citações

Classificação Científica

FOS: Ciências da engenharia e tecnologias > Engenharia química

CORDIS: Ciências Tecnológicas

Projetos Associados

LA 20 - Projeto Estratégico-LA 20 - 2011-2012

Outras Informações

ID Authenticus: P-002-R8A

DOI: 10.1007/s12010-010-9154-2

Abstract (EN): The activity and stability of laccase and their kinetic mechanisms in water soluble ionic liquids (ILs): 1-butyl-3-methyl imidazolium chloride [C(4)mim][Cl], 1-octyl-3-methyl imidazolium chloride [C(8)mim][Cl], and 1-decyl-3-methyl imidazolium chloride [C(10)mim][Cl] were investigated. The results show that an IL concentration up to 10% is satisfactory for initial laccase activity at pH 9.0. The laccase stability was well maintained in [C(4)mim][Cl] IL when compared to the control. The inactivation of laccase increases with the length of the alkyl chain in the IL: [C(10)mim][Cl] > [C(8)mim][Cl] > [C(4)mim][Cl]. The kinetic studies in the presence of ABTS as substrate allowed calculating the Michaelis-Menten parameters. Among the ILs, [C(4)mim][Cl] was the suitable choice attending to laccase activity and stability. Alkyl chains in the ions of ILs have a deactivating effect on laccase, which increases strongly with the length of the alkyl chain.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10