Study of the alkyl chain length on laccase stability and enzymatic kinetic with imidazolium ionic liquids

• Type: Article in International Scientific Journal
• Date: 2011

Authors

Oscar Rodríguez (Author) FEUP; Raquel O. Cristóvão (Author) FEUP; Ana P. M. Tavares (Author) FEUP; Eugénia A. Macedo (Author) FEUP

Journal

The Journal is awaiting validation by the Administrative Services.

Title: Appl Biochem Biotechnol - Applied Biochemistry and Biotechnology

Vol. 164 No. 4

Pages: 524-533

ISSN: 0273-2289

Indexing

ISI Web of Knowledge - 36 Citations

ISI Web of Science

Scopus - 39 Citations

Scientific classification

FOS: Engineering and technology > Chemical engineering

CORDIS: Technological sciences

Associated Projects

LA 20 - Projeto Estratégico-LA 20 - 2011-2012

Other information

Authenticus ID: P-002-R8A

DOI: 10.1007/s12010-010-9154-2

Abstract (EN): The activity and stability of laccase and their kinetic mechanisms in water soluble ionic liquids (ILs): 1-butyl-3-methyl imidazolium chloride [C(4)mim][Cl], 1-octyl-3-methyl imidazolium chloride [C(8)mim][Cl], and 1-decyl-3-methyl imidazolium chloride [C(10)mim][Cl] were investigated. The results show that an IL concentration up to 10% is satisfactory for initial laccase activity at pH 9.0. The laccase stability was well maintained in [C(4)mim][Cl] IL when compared to the control. The inactivation of laccase increases with the length of the alkyl chain in the IL: [C(10)mim][Cl] > [C(8)mim][Cl] > [C(4)mim][Cl]. The kinetic studies in the presence of ABTS as substrate allowed calculating the Michaelis-Menten parameters. Among the ILs, [C(4)mim][Cl] was the suitable choice attending to laccase activity and stability. Alkyl chains in the ions of ILs have a deactivating effect on laccase, which increases strongly with the length of the alkyl chain.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10