Analysis of aldehydes in beer by gas-diffusion microextraction: Characterization by high-performance liquid chromatography-diode-array detection-atmospheric pressure chemical ionization-mass spectrometry

• Tipo: Artigo em Revista Científica Internacional
• Data: 2010

Autores

Luis Moreira Goncalves (Autor) Outra; Paulo Jorge Magalhaes (Autor) Outra; Ines Maria Valente (Autor) Outra; Joao Grosso Pacheco (Autor) Outra; Pavel Dostalek (Autor) Outra; David Sykora (Autor) Outra; Jose Antonio Rodrigues (Autor) FCUP; Aquiles Araujo Barros (Autor) FCUP

Revista

Título: Journal of Chromatography A

Vol. 1217 Nº 43

Páginas: 3717-3722

ISSN: 0021-9673

Editora: Elsevier

Indexação

ISI Web of Knowledge - 45 Citações

ISI Web of Science

Scopus - 49 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

CORDIS: Ciências Físicas > Química > Química analítica > Espectroquímica ; Ciências Físicas > Química > Química aplicada > Química alimentar

Outras Informações

ID Authenticus: P-003-5QS

DOI: 10.1016/j.chroma.2010.04.002

Abstract (EN): In this work, a recently developed extraction technique for sample preparation aiming the analysis of volatile and semi-volatile compounds named gas-diffusion microextraction (GDME) is applied in the chromatographic analysis of aldehydes in beer. Aldehydes namely acetaldehyde (M), methylpropanal (MA) and furfural (FA) were simultaneously extracted and derivatized with 2,4-dinitrophenylhydrazine (DNPH), then the derivatives were separated and analyzed by high-performance liquid chromatography with spectrophotometric detection (HPLC-UV). The identity of the eluted compounds was confirmed by high-performance liquid chromatography-atmospheric pressure chemical ionization-mass-spectrometry detection in the negative ion mode (HPLC-APCI-MS). The developed methodology showed good repeatability (ca. 5%) and linearity as well as good limits of detection (AA-12.3. FA-1.5 and MA 5.4 mu gL(-1)) and quantification (AA-41, FA-4.9 and MA 181 mu gL(-1)); it also appears to be competitive in terms of speed and cost of analysis.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6