Analysis of aldehydes in beer by gas-diffusion microextraction: Characterization by high-performance liquid chromatography-diode-array detection-atmospheric pressure chemical ionization-mass spectrometry

• Type: Article in International Scientific Journal
• Date: 2010

Authors

Luis Moreira Goncalves (Author) Other; Paulo Jorge Magalhaes (Author) Other; Ines Maria Valente (Author) Other; Joao Grosso Pacheco (Author) Other; Pavel Dostalek (Author) Other; David Sykora (Author) Other; Jose Antonio Rodrigues (Author) FCUP; Aquiles Araujo Barros (Author) FCUP

Journal

Title: Journal of Chromatography A

Vol. 1217 No. 43

Pages: 3717-3722

ISSN: 0021-9673

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 45 Citations

ISI Web of Science

Scopus - 49 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

CORDIS: Physical sciences > Chemistry > Analytical chemistry > Spectrochemistry ; Physical sciences > Chemistry > Applied chemistry > Food chemistry

Other information

Authenticus ID: P-003-5QS

DOI: 10.1016/j.chroma.2010.04.002

Abstract (EN): In this work, a recently developed extraction technique for sample preparation aiming the analysis of volatile and semi-volatile compounds named gas-diffusion microextraction (GDME) is applied in the chromatographic analysis of aldehydes in beer. Aldehydes namely acetaldehyde (M), methylpropanal (MA) and furfural (FA) were simultaneously extracted and derivatized with 2,4-dinitrophenylhydrazine (DNPH), then the derivatives were separated and analyzed by high-performance liquid chromatography with spectrophotometric detection (HPLC-UV). The identity of the eluted compounds was confirmed by high-performance liquid chromatography-atmospheric pressure chemical ionization-mass-spectrometry detection in the negative ion mode (HPLC-APCI-MS). The developed methodology showed good repeatability (ca. 5%) and linearity as well as good limits of detection (AA-12.3. FA-1.5 and MA 5.4 mu gL(-1)) and quantification (AA-41, FA-4.9 and MA 181 mu gL(-1)); it also appears to be competitive in terms of speed and cost of analysis.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6