Multilayer platform to model the bioactivity of hyaluronic acid in gastric cancer

• Tipo: Artigo em Revista Científica Internacional
• Data: 2021

Autores

Amorim, S (Autor) Outra; da Costa, DS (Autor) Outra; Mereiter, S (Autor) Outra; Pashkuleva, I (Autor) Outra; Celso Reis (Autor) ICBAS; Rui Reis (Autor) Outra; Pires, RA (Autor) Outra

Revista

Título: MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS

Vol. 119

ISSN: 0928-4931

Editora: Elsevier

Indexação

ISI Web of Knowledge - 5 Citações

Scopus - 6 Citações

Outras Informações

ID Authenticus: P-00S-WPH

DOI: 10.1016/j.msec.2020.111616

Abstract (EN): Hyaluronic acid (HA) has a key role in cancer progression. The HA's molecular weight (M-w) is altered in this pathological state: increased concentration of shorter fragments due to the overexpressed hyaluronidases and ROS. Aiming to mimic this microenvironment, we developed a Layer-by-Layer (LbL) platform presenting HA of different M(w)s, namely 6.4, 752 and 1500 kDa, to study the influence of HA M-w on the formation of focal adhesion sites (FAs), and the involvement of paxillin and CD44 in this process. High paxillin expression and formation of FAs, via CD44, is observed for MKN45 cells seeded on LbLs presenting HA 6.4 kDa, with the activation of the ERK1/2 pathway, responsible for cell motility and tumour progression. In contrast, activation of p38 pathway, usually related with cancer latency, is observed for cells seeded on LbLs with high M-w HA, i.e. 1500 kDa. Overall, we demonstrate the suitability of the developed platform to study cancer invasiveness.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10