Multilayer platform to model the bioactivity of hyaluronic acid in gastric cancer

• Type: Article in International Scientific Journal
• Date: 2021

Authors

Amorim, S (Author) Other; da Costa, DS (Author) Other; Mereiter, S (Author) Other; Pashkuleva, I (Author) Other; Celso Reis (Author) ICBAS; Rui Reis (Author) Other; Pires, RA (Author) Other

Journal

Title: MATERIALS SCIENCE & ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS

Vol. 119

ISSN: 0928-4931

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 5 Citations

Scopus - 6 Citations

Other information

Authenticus ID: P-00S-WPH

DOI: 10.1016/j.msec.2020.111616

Abstract (EN): Hyaluronic acid (HA) has a key role in cancer progression. The HA's molecular weight (M-w) is altered in this pathological state: increased concentration of shorter fragments due to the overexpressed hyaluronidases and ROS. Aiming to mimic this microenvironment, we developed a Layer-by-Layer (LbL) platform presenting HA of different M(w)s, namely 6.4, 752 and 1500 kDa, to study the influence of HA M-w on the formation of focal adhesion sites (FAs), and the involvement of paxillin and CD44 in this process. High paxillin expression and formation of FAs, via CD44, is observed for MKN45 cells seeded on LbLs presenting HA 6.4 kDa, with the activation of the ERK1/2 pathway, responsible for cell motility and tumour progression. In contrast, activation of p38 pathway, usually related with cancer latency, is observed for cells seeded on LbLs with high M-w HA, i.e. 1500 kDa. Overall, we demonstrate the suitability of the developed platform to study cancer invasiveness.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10