Endurance training and chronic intermittent hypoxia modulate in vitro salicylate-induced hepatic mitochondrial dysfunction

• Tipo: Artigo em Revista Científica Internacional
• Data: 2012

Autores

ascensao, a (Autor) FADEUP; goncalves, io (Autor) Outra; lumini-oliveira, j (Autor) Outra; marques-aleixo, i (Autor) Outra; dos passos, e (Autor) Outra; rocha-rodrigues, s (Autor) Outra; machado, ng (Autor) Outra; moreira, ac (Autor) Outra; oliveira, pj (Autor) Outra; torrella, jr (Autor) Outra; magalhaes, j (Autor) FADEUP

Revista

Título: Mitochondrion

Vol. 12

Páginas: 607-616

ISSN: 1567-7249

Editora: Elsevier

Indexação

ISI Web of Knowledge - 12 Citações

Scopus - 12 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-002-3R1

DOI: 10.1016/j.mito.2012.10.007

Abstract (EN): Mitochondrial function is modulated by multiple approaches including physical activity, which can afford cross-tolerance against a variety of insults. We therefore aimed to analyze the effects of endurance-training (ET) and chronic-intermittent hypobaric-hypoxia (IHH) on liver mitochondrial bioenergetics and whether these effects translate into benefits against in vitro salicylate mitochondrial toxicity. Twenty-eight young-adult male rats were divided into normoxic-sedentary (NS), normoxic-exercised (NE), hypoxic-sedentary (HS) and hypoxic-exercised (HE). ET consisted of 1 h/days of treadmill running and IHH of simulated atmospheric pressure of 49.3 kPa 5 h/days during 5 weeks. Liver mitochondrial oxygen consumption, transmembrane-electric potential (Delta Psi) and permeability transition pore induction (MPTP) were evaluated in the presence and absence of salicylate. Aconitase, MnSOD, caspase-3 and 8 activities, - SH, MDA, SIRT3, Cyp D, HSP70, and OXPHOS subunit contents were assessed. ET and IHH decreased basal mitochondrial state-3 and state-4 respiration, although no alterations were observed in Delta Psi, endpoints evaluated in control mitochondria. In the presence of salicylate, ET and IHH decreased state-4 and lag-phase of ADP-phosphoiylation. Moreover, ADP-lag phase in hypoxic was further lower than in normoxic groups. Neither ET nor IHH altered the susceptibility to calcium-induced MPTP. IHH lowered MnSOD and increased aconitase activities. ET and IHH decreased caspase 8 activity whereas no effect was observed on caspase 3. The levels of SIRT3 increased with ET and IHH and Cyp D decreased with IHH. Data suggest that ET and IHH do not alter general basal liver mitochondrial function, but may attenuate some adverse effects of salicylate.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10