Simultaneous determination of E-2-nonenal and beta-damascenone in beer by reversed-phase liquid chromatography with UV detection

• Tipo: Artigo em Revista Científica Internacional
• Data: 2004

Autores

Guido, LF (Autor) FCUP; Carneiro, JR (Autor) Outra; Santos, JR (Autor) REIT; Almeida, PJ (Autor) FCUP; Rodrigues, JA (Autor) FCUP; Barros, AA (Autor) FCUP

Revista

Título: Journal of Chromatography A

Vol. 1032

Páginas: 17-22

ISSN: 0021-9673

Editora: Elsevier

Indexação

ISI Web of Knowledge - 30 Citações

Scopus - 30 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

Outras Informações

ID Authenticus: P-000-AVK

DOI: 10.1016/j.chroma.2003.12.026

Abstract (EN): A method for the simultaneous determination of E-2-nonenal and beta-damascenone in beer by reversed-phase liquid chromatography using UV detection is presented. The method consists of beer steam distillation, followed by an extraction/concentration step using Sep-Pak Plus C-18 RP cartridges and determination by HPLC at 226 nm UV-absorption maximum. The identity of the compounds was confirmed by GC analysis with MS detection of the isolated fractions. A recovery factor of approximately 80% was obtained for beta-damascenone with a R.S.D. of 3%. E-2-Nonenal and beta-damascenone were monitored in a comparative study of fresh and either naturally and forced aged beer. The results obtained show that both compounds have a similar behaviour through an extended storage of beer and consequently can be used as good analytical markers of beer ageing. Nevertheless, the use of beta-damascenone seems to be more convenient because this compound appears in beer in higher concentrations than E-2-nonenal, thus making it easier to measure.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6