Molecular and phylogenetic characterization of potentially toxic cyanobacteria in Tunisian freshwaters

• Tipo: Artigo em Revista Científica Internacional
• Data: 2011

Autores

Afef Fathalli (Autor) Outra; Amel Ben Rejeb Jenhani (Autor) Outra; Cristiana Moreira (Autor) Outra; Martin Welker (Autor) Outra; Mohamed Romdhane (Autor) Outra; Agostinho Antunes (Autor) FCUP; Vitor Vasconcelos (Autor) FCUP

Revista

Título: Systematic and Applied Microbiology

Vol. 34

Páginas: 303-310

ISSN: 0723-2020

Editora: Elsevier

Indexação

ISI Web of Knowledge - 34 Citações

Scopus - 37 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-002-QS1

DOI: 10.1016/j.syapm.2010.12.003

Abstract (EN): This study presents a genetic characterization of 27 potentially toxic cyanobacterial strains isolated from seven reservoirs located in the north and centre of Tunisia. These strains belonged mainly to Microcystis aeruginosa, Cylindrospermopsis raciborskii and Planktothrix agardhii species. Their toxicological potential was evaluated by molecular biology tools, which showed that none of the isolated strains carried segments of the gene cluster responsible for the production of cylindrospermopsin and saxitoxin. The majority of Microcystis isolates were able to synthesize microcystin, since they presented the six characteristic segments of the microcystin synthetase mcy cluster (mcyA, -B, -C, -D, -E and -G). This was further confirmed by MALDI-TOF analysis that showed the presence of eight microcystin variants, including microcystin-LR. The taxonomic identification of the strains was assessed based on the variability of the 16S rRNA gene sequences. Furthermore, the 16S-23S rRNA ITS sequences of Microcystis isolates and rpoC1 sequences of Cylindrospermopsis strains were also used in the phylogenetic analysis.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8