Characterization and transcriptional analysis of hupSLW in Gloeothece sp ATCC 27152: an uptake hydrogenase from a unicellular cyanobacterium

• Tipo: Artigo em Revista Científica Internacional
• Data: 2004

Autores

Oliveira, P (Autor) Outra; Leitao, E (Autor) Outra; Tamagnini, P (Autor) FCUP; Moradas Ferreira, P (Autor) ICBAS; Oxelfelt, F (Autor) Outra

Revista

Título: Microbiology

Vol. 150

Páginas: 3647-3655

ISSN: 1350-0872

Editora: Microbiological Society

Indexação

ISI Web of Knowledge - 32 Citações

Scopus - 32 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-000-7ZB

DOI: 10.1099/mic.0.27248-0

Abstract (EN): The structural genes (hupSL) encoding an uptake hydrogenase in the unicellular cyanobacterium Gloeothece sp. ATCC 27152, a strain capable of aerobic N-2 fixation, were identified and sequenced, 3'-RACE experiments uncovered the presence of an additional ORF 184 bp downstream of hupL, showing a high degree of sequence identity with a gene encoding an uptake- hydrogenase-specific enclopepticlase (hupW) in other cyanobacteria. In addition, the transcription start point was identified 238 bp upstream of the hupS translational start. RT-PCR experiments revealed that hupW is co-transcribed with the uptake hydrogenase structural genes in Gloeothece sp. ATCC 27152. In addition, Northern hybridizations clearly showed that hupSL Ware transcribed under nitrogen fixing conditions, but not in the presence of combined nitrogen. A putative NtcA binding site was identified in the promoter region upstream of hupS, centred at -41(.)5 bp with respect to the transcription start point. Electrophoretic retardation of a labelled DNA fragment (harbouring the putative NtcA-binding motif) was significantly affected by an Escherichia coli cell-free extract containing overexpressed NtcA, suggesting that NtcA is involved in the transcriptional regulation of hupSLW.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9