Light-activated amino-substituted dyes as dual-action antibacterial agents: Bio-efficacy and AFM evaluation

• Tipo: Artigo em Revista Científica Internacional
• Data: 2024

Autores

Correia, P (Autor) Outra; Araújo, P (Autor) Outra; Plácido, A (Autor) Outra; Pereira, AR (Autor) Outra; Bessa, LJ (Autor) Outra; Nuno Mateus (Autor) FCUP; Victor de Freitas (Autor) FCUP; Oliveira, J (Autor) Outra; Iva Fernandes (Autor) FCUP

Revista

Título: Dyes and Pigments

Vol. 224

ISSN: 0143-7208

Editora: Elsevier

Indexação

ISI Web of Knowledge - 0 Citações

Scopus - 0 Citações

Outras Informações

ID Authenticus: P-00Z-T3G

DOI: 10.1016/j.dyepig.2024.111975

Abstract (EN): The present study aimed to explore the antibacterial activity of a group of nature-inspired and structurally similar amino-based flavylium dyes: 7-diethylamino-4 '- dimethylaminoflavylium (7NEt24 ' NMe2); 7-diethylamino-2-(dimethylaminostyryl)-1-benzopyrylium (7NEt2st4 ' NMe2); 7-diethylamino-4 '-aminoflavylium (7NEt24 ' NH2); 7-diethylamino-4 '-hydroxyflavylium (7NEt24 ' OH). Except for 7NEt24 ' OH, all the other tested compounds showed inhibitory activity on the bacterial growth of Gram -positive bacteria (Staphylococcus aureus ATCC 29213 and S. epidermidis ATCC 14990) and Gram-negative bacteria (Pseudomonas aeruginosa ATCC 27853), with MIC values in the range of 2.61-24.4 mu M and 359-669 mu M, respectively. Besides their toxicity in the dark, remarkable light-induced killing effects were observed (light dose treatment of 22.5 J/cm2), causing significant morphological changes detected by atomic force microscopy. The photoinactivation effect was particularly effective against Gram -positive bacteria, where complete eradication of bacterial growth was achieved at concentrations in the range of 3-12 mu M. Photochemical mechanistic studies suggested the occurrence of both type I (free radicals) and type II (singlet oxygen) reactions, although light-induced damages appear to be predominantly caused by singlet oxygen action. Altogether, these results highlight the dual-antibacterial action of this group of dyes, showing antibiotic activity in the dark and photosensitizer behavior under visible light exposure. These findings underline a new application for amino-based flavylium dyes in the management of microbial skin infections.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 15