The role of ligand-gated conformational changes in enzyme catalysis

• Tipo: Artigo em Revista Científica Internacional
• Data: 2019

Autores

Moreira, C (Autor) Outra; Calixto, AR (Autor) Outra; Richard, JP (Autor) Outra; Kamerlin, SCL (Autor) Outra

Revista

Título: Biochemical Society Transactions

Vol. 47

Páginas: 1449-1460

ISSN: 0300-5127

Editora: PORTLAND PRESS LTD

Outras Informações

ID Authenticus: P-00Y-72X

DOI: 10.1042/bst20190298

Abstract (EN): <jats:title>Abstract</jats:title> <jats:p>Structural and biochemical studies on diverse enzymes have highlighted the importance of ligand-gated conformational changes in enzyme catalysis, where the intrinsic binding energy of the common phosphoryl group of their substrates is used to drive energetically unfavorable conformational changes in catalytic loops, from inactive open to catalytically competent closed conformations. However, computational studies have historically been unable to capture the activating role of these conformational changes. Here, we discuss recent experimental and computational studies, which can remarkably pinpoint the role of ligand-gated conformational changes in enzyme catalysis, even when not modeling the loop dynamics explicitly. Finally, through our joint analyses of these data, we demonstrate how the synergy between theory and experiment is crucial for furthering our understanding of enzyme catalysis.</jats:p>

Idioma: Inglês

Tipo (Avaliação Docente): Científica