Biomolecular Fishing: Design, Green Synthesis, and Performance of L-Leucine-Molecularly Imprinted Polymers

• Tipo: Artigo em Revista Científica Internacional
• Data: 2023

Autores

Furtado, AI (Autor) Outra; Viveiros, R (Autor) Outra; Bonifacio, VDB (Autor) Outra; Andre Melo (Autor) FCUP; Casimiro, T (Autor) Outra

Revista

Título: ACS Omega

Vol. 8

Páginas: 9179-9186

Editora: American Chemical Society

Indexação

ISI Web of Knowledge - 0 Citações

Scopus

Outras Informações

ID Authenticus: P-00Y-2RV

DOI: 10.1021/acsomega.2c05714

Abstract (EN): Biopurification is a challenging and growing market. Despite great efforts in the past years, current purification strategies still lack specificity, efficiency, and cost-effectiveness. The development of more sustainable functional materials and processes needs to address pressing environmental goals, efficiency, scale-up, and cost. Herein, L-leucine (LEU)-molecularly imprinted polymers (MIPs), LEU-MIPs, are presented as novel biomolecular fishing polymers for affinity sustainable biopurification. Rational design was performed using quantum mechanics calculations and molecular modeling for selecting the most appropriate monomers. LEU-MIPs were synthesized for the first time by two different green approaches, supercritical carbon dioxide (scCO(2)) technology and mechanochemistry. A significant imprinting factor of 12 and a binding capacity of 27 mg LEU/g polymer were obtained for the LEU-MIP synthesized in scCO(2) using 2-vinylpyridine as a functional monomer, while the LEU-MIP using acrylamide as a functional monomer synthesized by mechanochemistry showed an imprinting factor of 1.4 and a binding capacity of 18 mg LEU/g polymer, both systems operating at a low binding concentration (0.5 mg LEU/mL) under physiological conditions. As expected, at a higher concentration (1.5 mg LEU/mL), the binding capacity was considerably increased. Both green technologies show high potential in obtaining ready-to-use, stable, and low-cost polymers with a molecular recognition ability for target biomolecules, being promising materials for biopurification processes.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8