Development of a Screening Method for Sulfamethoxazole in Environmental Water by Digital Colorimetry Using a Mobile Device

• Tipo: Artigo em Revista Científica Internacional
• Data: 2022

Autores

Peixoto, PS (Autor) Outra; Carvalho, PH (Autor) Outra; Machado, A (Autor) Outra; Barreiros, L (Autor) Outra; Bordalo, AA (Autor) Outra; Oliveira, HP (Autor) FCUP; Marcela A Segundo (Autor) FFUP

Revista

A Revista está pendente de validação pelos Serviços Administrativos.

Título: Chemosensors

Vol. 10

Página Final: 25

ISSN: 2227-9040

Indexação

ISI Web of Knowledge - 0 Citações

Scopus - 1 Citação

Outras Informações

ID Authenticus: P-00V-YE3

DOI: 10.3390/chemosensors10010025

Abstract (EN): Antibiotic resistance is a major health concern of the 21st century. The misuse of antibiotics over the years has led to their increasing presence in the environment, particularly in water resources, which can exacerbate the transmission of resistance genes and facilitate the emergence of resistant microorganisms. The objective of the present work is to develop a chemosensor for screening of sulfonamides in environmental waters, targeting sulfamethoxazole as the model analyte. The methodology was based on the retention of sulfamethoxazole in disks containing polystyrene divinylbenzene sulfonated sorbent particles and reaction with p-dimethylaminocinnamaldehyde, followed by colorimetric detection using a computer-vision algorithm. Several color spaces (RGB, HSV and CIELAB) were evaluated, with the coordinate a_star, from the CIELAB color space, providing the highest sensitivity. Moreover, in order to avoid possible errors due to variations in illumination, a color palette is included in the picture of the analytical disk, and a correction using the a_star value from one of the color patches is proposed. The methodology presented recoveries of 82-101% at 0.1 mu g and 0.5 mu g of sulfamethoxazole (25 mL), providing a detection limit of 0.08 mu g and a quantification limit of 0.26 mu g. As a proof of concept, application to in-field analysis was successfully implemented.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 12