The population-level impact of Enterococcus faecalis genetics on intestinal colonisation and extraintestinal infection

• Tipo: Outra Publicação em Revista Científica Internacional
• Data: 2022

Autores

Chaguza, C (Autor) Outra; Pöntinen, AK (Autor) Outra; Top, J (Autor) Outra; Arredondo Alonso, S (Autor) Outra; Freitas, AR (Autor) Outra; Novais, Carla (Autor) FFUP; Torres, C (Autor) Outra; Bentley, SD (Autor) Outra; Luisa Peixe (Autor) FFUP; Coque, TM (Autor) Outra; Willems, RJ (Autor) Outra; Corander, J (Autor) Outra

Outras Informações

ID Authenticus: P-00Y-3F0

DOI: 10.1101/2022.09.26.509451

Abstract (EN): <jats:title>Abstract</jats:title><jats:p><jats:italic>Enterococcus faecalis</jats:italic> is a commensal pathogenic bacterium commonly found in the human gastrointestinal tract and a cause of opportunistic infections typically associated with multidrug resistance. The <jats:italic>E. faecalis</jats:italic> genetic changes associated with pathogenicity and extraintestinal infection, particularly through gut-to-bloodstream translocation, are poorly understood. Here, we investigate the <jats:italic>E. faecalis</jats:italic> genetic signatures associated with intestinal colonisation and extraintestinal infection and infection of hospitalised and non-hospitalised individuals using heritability estimation and a genome-wide association study (GWAS). We analysed 750 whole-genome sequences of faecal and bloodstream <jats:italic>E. faecalis</jats:italic> isolates from hospitalised patients and non-hospitalised individuals, respectively, predominantly in Europe. We found that <jats:italic>E. faecalis</jats:italic> infection of individuals depending on their hospitalisation status and extraintestinal infection are heritable traits and that ~24% and ~34% of their variation is explained by the considered genetic effects, respectively. Further, a GWAS using linear mixed models did not pinpoint any clear enrichment of individual genetic changes in isolates from different isolation sites and individuals with varying hospitalisation statuses, suggesting that these traits are highly polygenic. Altogether, our findings indicate that <jats:italic>E. faecalis</jats:italic> infection and extraintestinal infection are influenced by variation in genetic, host, and environmental factors, and ultimately the opportunistic pathogenic lifestyle of this versatile host generalist bacterium.</jats:p>

Idioma: Inglês

Tipo (Avaliação Docente): Científica