Green coconut fiber: a novel carrier for the immobilization of commercial laccase by covalent attachment for textile dyes decolourization

• Tipo: Artigo em Revista Científica Internacional
• Data: 2012

Autores

Raquel O. Cristóvão (Autor) FEUP; Sara C. Silvério (Autor) Outra; Ana P. M. Tavares (Autor) FEUP; Ana Iraidy S. Brígida (Autor) Outra; José M. Loureiro (Autor) FEUP; Rui A. R. Boaventura (Autor) FEUP; Eugénia A. Macedo (Autor) FEUP; Maria Alice Z. Coelho (Autor) Outra

Revista

Título: World Journal of Microbiology and Biotechnology

Vol. 28 Nº 9

Páginas: 2827-2838

ISSN: 0959-3993

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 12 Citações

ISI Web of Science

Scopus - 12 Citações

Classificação Científica

FOS: Ciências da engenharia e tecnologias > Engenharia do ambiente

CORDIS: Ciências Tecnológicas > Tecnologia > Tecnologia ambiental

Projetos Associados

LA 20 - Projeto Estratégico-LA 20 - 2011-2012

Outras Informações

ID Authenticus: P-00H-537

DOI: 10.1007/s11274-012-1092-4

Abstract (EN): Commercial laccase formulation was immobilized on modified green coconut fiber silanized with 3-glycidoxypropyltrimethoxysilane, aiming to achieve a cheap and effective biocatalyst. Two different strategies were followed: one point (pH 7.0) and multipoint (pH 10.0) covalent attachment. The influence of immobilization time on enzymatic activity and the final reduction with sodium borohydride were evaluated. The highest activities were achieved after 2 h of contact time in all situations. Commercial laccase immobilized at pH 7.0 was found to have higher activity and higher affinity to the substrate. However, the immobilization by multipoint covalent attachment improved the biocatalyst thermal stability at 50 A degrees C, when compared to soluble enzyme and to the immobilized enzyme at pH 7.0. The Schiff's bases reduction by sodium borohydride, in spite of causing a decrease in enzyme activity, showed to contribute to the increase of operational stability through bonds stabilization. Finally, these immobilized enzymes showed high efficiency in the continuous decolourization of reactive textile dyes. In the first cycle, the decolourization is mainly due to dyes adsorption on the support. However, when working in successive cycles, the adsorption capacity of the support decreases (saturation) and the enzymatic action increases, indicating the applicability of this biocatalyst for textile wastewater treatment.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 12