High-Resolution Analysis of Centrosome Behavior During Mitosis

• Tipo: Capítulo ou Parte de Livro
• Data: 2021

Autores

Nunes, V (Autor) Outra; Dantas, M (Autor) Outra; Lima, JT (Autor) Outra; J Ferreira (Autor) FMUP

Indexação

Scopus - 0 Citações

Outras Informações

ID Authenticus: P-00V-1Z1

DOI: 10.1007/978-1-0716-1538-6_13

Abstract (EN): Cell division requires a dynamic reorganization of cytoskeletal and nuclear components. One essential step is the separation of centrosomes, which allows the assembly of a microtubule-based mitotic spindle. This has to be spatially and temporally coordinated with other events such as adhesion complex disengagement, assembly of an actin-rich cell cortex and nuclear envelope breakdown (NEB), to ensure chromosome segregation fidelity. Previous methodologies often focused on a single event and failed to provide an integrated view of the process. In this chapter, we describe a method to study mitosis with high resolution, by analyzing the dynamic interplay between centrosomes, nucleus, and cell membrane, using a combination of live-cell imaging and micromanipulation with custom-designed computational tools. © 2021, Springer Science+Business Media, LLC, part of Springer Nature.

Idioma: Inglês

Tipo (Avaliação Docente): Científica