Species association increases biofilm resistance to chemical and mechanical treatments

• Tipo: Artigo em Revista Científica Internacional
• Data: 2009

Autores

Manuel Simoes (Autor) FEUP; Lucia C Simoes (Autor) Outra; Maria J Vieira (Autor) Outra

Revista

Título: Water Research

Vol. 43

Páginas: 229-237

ISSN: 0043-1354

Editora: Elsevier

Indexação

ISI Web of Knowledge - 86 Citações

ISI Web of Science

Scopus - 109 Citações

COMPENDEX

Classificação Científica

FOS: Ciências da engenharia e tecnologias > Engenharia do ambiente

CORDIS: Ciências Tecnológicas > Engenharia

Outras Informações

ID Authenticus: P-003-SCP

DOI: 10.1016/j.watres.2008.10.010

Abstract (EN): The study of biofilm ecology and interactions might help to improve our understanding of their resistance mechanisms to control strategies. Concerns that the diversity of the biofilm communities can affect disinfection efficacy have led us to examine the effect of two antimicrobial agents on two important spoilage bacteria. Studies were conducted on single and dual species biofilms of Bacillus cereus and Pseudomonas fluorescens. Biofilms were formed on a stainless steel rotating device, in a bioreactor, at a constant Reynolds number of agitation (Re(A)). Biofilm phenotypic characterization showed significant differences, mainly in the metabolic activity and both extracellular proteins and polysaccharides content. Cetyl trimethyl ammonium bromide (CTAB) and glutaraldehyde (GLUT) solutions in conjunction with increasing ReA were used to treat biofilms in order to assess their ability to kill and remove biofilms. B. cereus and P. fluorescens biofilms were stratified in a layered structure with each layer having differential tolerance to chemical and mechanical stresses. Dual species biofilms and P. fluorescens single biofilms had both the highest resistance to removal when pre-treated with CTAB and GLUT, respectively. B. cereus biofilms were the most affected by hydrodynamic disturbance and the most susceptible to antimicrobials. Dual biofilms were more resistant to antimicrobials than each single species biofilm, with a significant proportion of the population remaining in a viable state after exposure to CTAB or GLUT. Moreover, the species association increased the proportion of viable cells of both bacteria, comparatively to the single species scenarios, enhancing each other's survival to antimicrobials and the biofilm shear stress stability.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9