A passive direct methanol fuel cell as transducer of an electrochemical sensor, applied to the detection of carcinoembryonic antigen

• Tipo: Artigo em Revista Científica Internacional
• Data: 2021-03-01

Autores

Liliana Carneiro (Autor) Outra; Nádia Ferreira (Autor) Outra; Ana Tavares (Autor) Outra; A.M.F.R. Pinto (Autor) FEUP; Adélio Mendes (Autor) FEUP; Goreti Sales (Autor) Outra

Revista

Título: Biosensors and Bioelectronics

Vol. 175

Página Inicial: 112877

ISSN: 0956-5663

Editora: Elsevier

Indexação

ISI Web of Knowledge - 0 Citações

Scopus - 5 Citações

Classificação Científica

CORDIS: Ciências Físicas > Química

FOS: Ciências da engenharia e tecnologias > Engenharia química

Outras Informações

ID Authenticus: P-00T-6MA

DOI: 10.1016/j.bios.2020.112877

Abstract (EN): This work describes an electrochemical sensor with a biomimetic plastic antibody film for carcinoembryonic antigen (CEA, an important biomarker in colorectal cancer), integrated in the electrical circuit of a direct methanol fuel cell (DMFC), working in passive mode and used herein as power supply and signal transducer. In detail, the sensing layer for CEA consisted of a Fluorine-doped Tin Oxide (FTO) conductive glass substrate - connected to the negative pole side of the DMFC - with a conductive poly (3,4-ethylenedioxythiophene) (PEDOT) layer and a polypyrml (PPy) molecularly-imprinted polymer (MIP), assembled in-situ. This sensing element is then closed using a cover FTO-glass, hold in place with a clip, connected to the positive side of the DMFC. When compared with control DMFCs, the power curves of DMFC/Sensor integrated system showed decreased power values due to the MIP layer interfaced in the electrical circuit, also displaying high stability signals. The DMFC/Sensor was further calibrated at room temperature, in different medium (buffer, a synthetic physiological fluid model and Cormay (R) serum), showing linear responses over a wide concentration range, with a limit of detection of 0.08 ng/mL. The DMFC/Sensor presented sensitive data, with linear responses from 0.1 ng/mL to 100 mu g/mL and operating well in the presence of human serum. Overall, the results obtained evidenced the possibility of using a DMFC as a transducing element in an electrochemical sensor, confirming the sensitive and selective readings of the bio (sensing) imprinted film. This integration paves the way towards fully autonomous electrochemical devices, in which the integration of the sensor inside the fuel cell may be a subsequent direction.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9