Differences for T-Cell Subtypes in Aspiration Biopsies of Patients With Kidney Transplant Under Polyclonal and Monoclonal I mmunosuppressive Treatments

• Tipo: Artigo em Revista Científica Internacional
• Data: 2019

Autores

Xavier, P (Autor) Outra; Magalhaes, M (Autor) Outra; Sampaio Norton, S (Autor) FMUP; Guimaraes, T (Autor) Outra; José Oliveira (Autor) FMUP

Revista

Título: Transplantation Proceedings

Vol. 51

Páginas: 3271-3274

ISSN: 0041-1345

Editora: Elsevier

Indexação

ISI Web of Knowledge - 0 Citações

Scopus - 0 Citações

Outras Informações

ID Authenticus: P-00R-DE9

DOI: 10.1016/j.transproceed.2019.07.031

Abstract (EN): Thymoglobulin, or antithymocyte globulin (ATG), and anti-interleukin 2 alpha (IL-2 alpha) chain receptor antibody (IL-2 alpha RAb) achieve comparable good results in kidney transplantation notwithstanding different actions on immune cells. Previously, we reported the usefulness of flow cytometry (FC) analysis of lymphocyte subsets present in peripheral blood sample (PBL) and fine-needle aspiration biopsies (FNABs) for clinical surveillance, as, FC reaches very high predictive positive values for acute rejection diagnosis. Now we report an FC study on 2 kidney transplantation (KT) groups under ATG (n = 19) and IL-2 alpha RAb (n = 24) treatment. Both groups were further treated with calcineurin inhibitors mycophenolate mofetil (MMF) and prednisone. PBL and FNAB samples were collected on day 7 post-KT, stained for several T- and B-lymphocyte subsets, and acquired using FACScan. Statistical analysis were done by Mann-Whitney U test. FNAB results showed a significant downregulation by ATG of CD3 (P <.001), CD4 (P =.009), CD4CD29 (P =.003), and CD2 (P <=.001) and significant upregulation of death receptor (DR) (P =.03), CD3CD69 (P <.001), and CD3CD25 (P <.0001) as compared to groups treated with IL-2 alpha RAb. For PBL, the same trend was seen for CD3, CD4, CD2, CD3CD25, CD3CD69, CD4CD29, and DR plus a downregulation of CD45RO (P =.001) and an upregulation of CD4CD45RA (P <.0001) in IL-2 alpha RAb. This study shows that among stable KTs, ATG as compared to IL-2 alpha RAb induces a significant downregulation of a subset of T-memory (CD4CD29) cells but an upregulation of antigen-experienced cells (CD45RO). Further, ATG decreases CD2, CD3, CD4, and na ve (CD45RA) and stimulates T cells as translated by CD3CD69 and DR. As it should be expected from an IL-2 alpha RAb agent, CD25 cells were virtually eliminated.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 4