Two alternative pathways for docosahexaenoic acid (DHA, 22:6n-3) biosynthesis are widespread among teleost fish

• Tipo: Artigo em Revista Científica Internacional
• Data: 2017

Autores

Oboh, A (Autor) Outra; Kabeya, N (Autor) Outra; Carmona Antonanzas, G (Autor) Outra; Filipe F C Castro (Autor) FCUP; Dick, JR (Autor) Outra; Tocher, DR (Autor) Outra; Monroig, O (Autor) Outra

Revista

Título: Scientific Reports

Vol. 7

ISSN: 2045-2322

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 93 Citações

Scopus - 96 Citações

Outras Informações

ID Authenticus: P-00R-ATM

DOI: 10.1038/s41598-017-04288-2

Abstract (EN): Docosahexaenoic acid (DHA) plays important physiological roles in vertebrates. Studies in rats and rainbow trout confirmed that DHA biosynthesis proceeds through the so-called "Sprecher pathway", a biosynthetic process requiring a Delta 6 desaturation of 24::5n-3 to 24:6n-3. Alternatively, some teleosts possess fatty acyl desaturases 2 (Fads2) that enable them to biosynthesis DHA through a more direct route termed the "Delta 4 pathway". In order to elucidate the prevalence of both pathways among teleosts, we investigated the Delta 6 ability towards C-24 substrates of Fads2 from fish with different evolutionary and ecological backgrounds. Subsequently, we retrieved public databases to identify Fads2 containing the YXXN domain responsible for the Delta 4 desaturase function, and consequently enabling these species to operate the Delta 4 pathway. We demonstrated that, with the exception of Delta 4 desaturases, fish Fads2 have the ability to operate as Delta 6 desaturases towards C-24 PUFA enabling them to synthesise DHA through the Sprecher pathway. Nevertheless, the Delta 4 pathway represents an alternative route in some teleosts and we identified the presence of putative Delta 4 Fads2 in a further 11 species and confirmed the function as Delta 4 desaturases of Fads2 from medaka and Nile tilapia. Our results demonstrated that two alternative pathways for DHA biosynthesis exist in teleosts.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10