The Chemopreventive Effect of the Dietary Compound Kaempferol on the MCF-7 Human Breast Cancer Cell Line Is Dependent on Inhibition of Glucose Cellular Uptake

• Tipo: Artigo em Revista Científica Internacional
• Data: 2015

Autores

Azevedo, C (Autor) Outra; Correia Branco, A (Autor) Outra; Araújo JR (Autor) FMUP; guimaraes, jt (Autor) FMUP; Elisa Keating (Autor) FMUP; Martel, F (Autor) FMUP

Revista

Título: Nutrition and Cancer

Vol. 67

Páginas: 504-513

ISSN: 0163-5581

Editora: Taylor & Francis

Indexação

ISI Web of Knowledge - 95 Citações

Scopus - 100 Citações

Outras Informações

ID Authenticus: P-00A-AFX

DOI: 10.1080/01635581.2015.1002625

Abstract (EN): Our aim was to investigate the effect of several dietary polyphenols on glucose uptake by breast cancer cells. Uptake of H-3-deoxy-D-glucose (H-3-DG) by MCF-7 cells was time-dependent, saturable, and inhibited by cytochalasin B plus phloridzin. In the short-term (26min), myricetin, chrysin, genistein, resveratrol, kaempferol, and xanthohumol (10-100 mu M) inhibited H-3-DG uptake. Kaempferol was found to be the most potent inhibitor of H-3-DG uptake [IC50 of 4 mu M (1.6-9.8)], behaving as a mixed-type inhibitor. In the long-term (24h), kaempferol (30 mu M) was also able to inhibit H-3-DG uptake, associated with a 40% decrease in GLUT1 mRNA levels. Interestingly enough, kaempferol (100 mu M) revealed antiproliferative (sulforhodamine B and H-3-thymidine incorporation assays) and cytotoxic (extracellular lactate dehydrogenase activity determination) properties, which were mimicked by low extracellular (1mM) glucose conditions and reversed by high extracellular (20mM) glucose conditions. Finally, exposure of cells to kaempferol (30 mu M) induced an increase in extracellular lactate levels over time (to 731 +/- 32% of control after a 24 h exposure), due to inhibition of MCT1-mediated lactate cellular uptake. In conclusion, kaempferol potently inhibits glucose uptake by MCF-7 cells, apparently by decreasing GLUT1-mediated glucose uptake. The antiproliferative and cytotoxic effect of kaempferol in these cells appears to be dependent on this effect.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10