Xanthohumol inhibits cell proliferation and induces apoptosis in human thyroid cells

• Tipo: Artigo em Revista Científica Internacional
• Data: 2018

Autores

Carvalho, DO (Autor) FCUP; Freitas, J (Autor) Outra; Nogueira, P (Autor) Outra; Henriques, SN (Autor) Outra; Carmo, AM (Autor) Outra; Castro, MA (Autor) Outra; Luis F Guido (Autor) FCUP

Revista

Título: Food and Chemical Toxicology

Vol. 121

Páginas: 450-457

ISSN: 0278-6915

Editora: Elsevier

Indexação

ISI Web of Knowledge - 15 Citações

Scopus - 15 Citações

Outras Informações

ID Authenticus: P-00P-NSA

DOI: 10.1016/j.fct.2018.09.021

Abstract (EN): The cell growth inhibitory potential of xanthohumol (XN), a natural prenylflavonoid present in hops and beer, on human papillary thyroid cancer cells is reported. We demonstrate that XN decreases the proliferation of TPC-1 cancer cells in a dose and time dependent manners. At low concentration (10 mu M) XN was shown to significantly inhibit carcinogenesis by a mechanism that stops or slows down cell division, preserving the viability of the cells. At higher concentration (100 mu M) a decrease of cell viability was observed by induction of apoptosis. As evidenced, XN induced DNA fragmentation in TPC-1 cells and promoted cell cycle arrest, which decreased the percentage of cells in G1 phase and increased in S phase after 72 h of treatment. Furthermore, XN exposure triggered an increase in caspase-3 and caspase-7 activity, supporting its role in the activation of apoptosis. Cell free studies demonstrated that high concentrations of XN are responsible for an increase of free radicals generated in a Fenton system which may mediate apoptosis through a pro-oxidant pathway. Altogether, our data show that XN induces the apoptosis of TPC-1 cancer cells in a concentration-dependent manner, suggesting XN to be a promising candidate for thyroid cancer therapy.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8