New beta-galactosidase producers with potential for prebiotic synthesis

• Tipo: Artigo em Revista Científica Internacional
• Data: 2018

Autores

Eugénia A. Macedo (Autor) FEUP; Sara C. Silvério (Autor) Outra; José A. Teixeira (Autor) Outra; Lígia R. Rodrigues (Autor) Outra

Revista

Título: Bioresource Technology

Vol. 250

Páginas: 131-139

ISSN: 0960-8524

Editora: Elsevier

Indexação

ISI Web of Science

Scopus

Classificação Científica

CORDIS: Ciências Tecnológicas

FOS: Ciências da engenharia e tecnologias

Outras Informações

DOI: 10.1016/j.biortech.2017.11.045

Abstract (EN): beta-Galactosidases (EC 3.2.1.23) are interesting enzymes with potential application in the pharmaceutical and food industry. In this work, a screening study was carried out to identify new fungal sources of beta-galactosidase. A total of 50 fungi were evaluated using a chromogenic test performed in agar plates. The most promising microorganisms were validated as effective beta-galactosidase producers under submerged fermentation conditions. The crude beta-galactosidases were characterized regarding their optimal pH (3.0-5.5) and temperature (45-65 degrees C). All enzymes showed ability to synthesize lactose-based prebiotics, namely lactulose (maximal yield 3.3%) and a galacto-oligosaccharide (GOS) (maximal yield 20%). Additionally, some enzymatic extracts with fructosyltransferase activity allowed to produce other type of prebiotics, namely fructo-oligosaccharides (FOS). This work, reports for the first time the simultaneous synthesis of different mixtures of GOS (2-15% yield and 0.07-0.5 g/L.h(-1) productivity) and FOS (4-30% yield and 0.1-1 g/L.h(-1) productivity) by crude extracts exhibiting dual enzymatic activity.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9