Unravelling the genome of a Pseudomonas aeruginosa isolate belonging to the high-risk clone ST235 reveals an integrative conjugative element housing a bla(GES-6) carbapenemase

• Tipo: Artigo em Revista Científica Internacional
• Data: 2018

Autores

Botelho, J (Autor) Outra; Grosso, F (Autor) Outra; Luisa Peixe (Autor) FFUP

Revista

Título: Journal of Antimicrobial Chemotherapy

Vol. 73

Páginas: 77-83

ISSN: 0305-7453

Editora: Oxford University Press

Indexação

ISI Web of Knowledge - 10 Citações

Scopus - 15 Citações

Outras Informações

ID Authenticus: P-00N-FHA

DOI: 10.1093/jac/dkx337

Abstract (EN): Objectives: In Pseudomonas aeruginosa, the bla(GES-6) carbapenemase gene was previously associated with an In1076 class I integron. Here, we conducted a genome-based analysis and explored the genetic platform associated with the mobility of this gene. Methods: WGS of a bla(GES-6)-harbouring P. aeruginosa isolate (FFUP_PS_690) was performed with Illumina HiSeq, de novo assembly was performed using SPAdes and subsequent bioinformatic analysis was performed concerning antibiotic resistance genes, virulence features and mobile genetic elements. Results: The FFUP_PS_690 isolate belongs to the ST235 high-risk clone and houses a novel integrative conjugative element (ICE), hereby named ICEPae690. This clc-like ICE comprises the bla(GES-6)-harbouring In1076 integron and specific modules. An ExoU island A variant was also identified. Conclusions: The presence of a 'hitch-hiking' bla(GES-6)-harbouring In1076 integron in an ICE and an exoU-carrying genomic island highlight the potential spread of these elements through conjugation and/or clonal expansion of the ST235 lineage.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 7