Application of flow cytometry for the identification of Staphylococcus epidermidis by peptide nucleic acid fluorescence in situ hybridization (PNA FISH) in blood samples

• Tipo: Artigo em Revista Científica Internacional
• Data: 2011

Autores

Azevedo, NF (Autor) FEUP; Jardim, T (Autor) Outra; Almeida, C (Autor) Outra; Cerqueira, L (Autor) Outra; Almeida, AJ (Autor) Outra; Rodrigues, F (Autor) Outra; Keevil, CW (Autor) Outra; Vieira, MJ (Autor) Outra

Revista

Título: Antonie van Leeuwenhoek, International Journal of General and Molecular Microbiology

Vol. 100

Páginas: 463-470

ISSN: 0003-6072

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 15 Citações

Scopus - 18 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Ciências biológicas

Outras Informações

ID Authenticus: P-002-MA4

DOI: 10.1007/s10482-011-9595-9

Abstract (EN): Staphylococcus epidermidis is considered to be one of the most common causes of nosocomial bloodstream infections, particularly in immune-compromised individuals. Here, we report the development and application of a novel peptide nucleic acid probe for the specific detection of S. epidermidis by fluorescence in situ hybridization. The theoretical estimates of probe matching specificity and sensitivity were 89 and 87%, respectively. More importantly, the probe was shown not to hybridize with closely related species such as Staphylococcus aureus. The method was subsequently successfully adapted for the detection of S. epidermidis in mixed-species blood cultures both by microscopy and flow cytometry.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8