Mucosal and systemic T cell response in mice intragastrically infected with Neospora caninum tachyzoites

• Tipo: Artigo em Revista Científica Internacional
• Data: 2013

Autores

Alexandra Correia (Autor) ICBAS; Pedro Ferreirinha (Autor) Outra; Amanda A Costa (Autor) Outra; Joana Dias (Autor) Outra; Joana Melo (Autor) Outra; Rita Costa (Autor) Outra; Adilia Ribeiro (Autor) Outra; Augusto Faustino (Autor) Outra; Luzia Teixeira (Autor) ICBAS; Antonio Rocha (Autor) ICBAS; Manuel Vilanova (Autor) ICBAS

Revista

Título: Veterinary Research

Vol. 44 Nº 1

Página Final: 69

ISSN: 0928-4249

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 16 Citações

Scopus - 18 Citações

Classificação Científica

FOS: Ciências agrárias > Ciências veterinárias

CORDIS: Ciências da Saúde > Ciências Médicas > Medicina Veterinária

Outras Informações

ID Authenticus: P-006-88Q

DOI: 10.1186/1297-9716-44-69

Abstract (EN): The murine model has been widely used to study the host immune response to Neospora caninum. However, in most studies, the intraperitoneal route was preferentially used to establish infection. Here, C57BL/6 mice were infected with N. caninum tachyzoites by the intragastric route, as it more closely resembles the natural route of infection through the gastrointestinal tract. The elicited T-cell mediated immune response was evaluated in the intestinal epithelium and mesenteric lymph nodes (MLN). Early upon the parasitic challenge, IL-12 production by conventional and plasmacytoid dendritic cells was increased in MLN. Accordingly, increased proportions and numbers of TCR alpha beta(+)CD8(+)IFN-gamma(+) lymphocytes were detected, not only in the intestinal epithelium and MLN, but also in the spleen of the infected mice. In this organ, IFN-gamma-producing TCR alpha beta(+)CD4(+) T cells were also found to increase in the infected mice, however later than CD8(+) T cells. Interestingly, splenic and MLN CD4(+)CD25(+) T cells sorted from infected mice presented a suppressive activity on in vitro T cell proliferation and cytokine production above that of control counterparts. These results altogether indicate that, by producing IFN-gamma, TCR alpha beta(+)CD8(+) cells contribute for local and systemic host protection in the earliest days upon infection established through the gastrointestinal tract. Nevertheless, they also provide substantial evidence for a parasite-driven reinforcement of T regulatory cell function which may contribute for parasite persistence in the host and might represent an additional barrier to overcome towards effective vaccination.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 13