Tunable layer-by-layer films containing hyaluronic acid and their interactions with CD44

• Tipo: Artigo em Revista Científica Internacional
• Data: 2020

Autores

Amorim, S (Autor) Outra; Pashkuleva, I (Autor) Outra; Celso Reis (Autor) ICBAS; Rui Reis (Autor) Outra; Pires, RA (Autor) Outra

Revista

Título: Journal of Materials Chemistry B

Vol. 8

Páginas: 3880-3885

ISSN: 2050-7518

Editora: Royal Society of Chemistry

Indexação

ISI Web of Knowledge - 22 Citações

Scopus - 22 Citações

Outras Informações

ID Authenticus: P-00S-3D7

DOI: 10.1039/d0tb00407c

Abstract (EN): We report on the development of layer-by-layer (LbL) constructs whose viscoelastic properties and bioactivity can be finely tuned by using polyanions of different size and/or crosslinking. As a polyanion we used hyaluronic acid (HA) - a multi-signaling biomolecule whose bioactivity depends on its molecular weight. We investigated the interplay between the mechanical properties of the LbL systems built using HA of different sizes and the specific HA-mediated biochemical interactions. We characterized the assembled materials and their interactions with CD44, the main HA receptor, by Quartz Crystal Microbalance with Dissipation (QCM-D), Surface Plasmon Resonance (SPR) and Atomic Force Microscopy (AFM). We observed that the presence of CD44 resulted in the disruption of the non-crosslinked multilayers, while crosslinked films remain stable and bind CD44 in a HA molecular weight and charge specific fashion.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 6