Transcriptomic Characterization of the South American Freshwater Stingray Potamotrygon motoro Venom Apparatus

• Tipo: Artigo em Revista Científica Internacional
• Data: 2018

Autores

Silva, F (Autor) Outra; Huang, Y (Autor) Outra; Yang, V (Autor) Outra; Mu, XD (Autor) Outra; Shi, Q (Autor) Outra; Agostinho Antunes (Autor) FCUP

Revista

Título: Toxins

Nº de Série 6 Vol. 10 Nº 252

Página Final: 544

ISSN: 2072-6651

Editora: MDPI

Indexação

ISI Web of Knowledge - 10 Citações

Scopus - 12 Citações

Outras Informações

ID Authenticus: P-00Q-1H3

DOI: 10.3390/toxins10120544

Abstract (EN): Venomous animals are found through a wide taxonomic range including cartilaginous fish such as the freshwater stingray Potamotrygon motoro occurring in South America, which can injure people and cause venom-related symptoms. Ensuring the efficacy of drug development to treat stingray injuries can be assisted by the knowledge of the venom composition. Here we performed a detailed transcriptomic characterization of the venom gland of the South American freshwater stingray Potamotrygon motoro. The transcripts retrieved showed 418 hits to venom components (comparably to 426 and 396 hits in other two Potamotrygon species), with high expression levels of hyaluronidase, cystatin and calglandulin along with hits uniquely found in P. motoro such as DELTA-alicitoxin-Pse1b, Augerpeptide hhe53 and PI-actitoxin-Aeq3a. We also identified undescribed molecules with extremely high expression values with sequence similarity to the SE-cephalotoxin and Rapunzel genes. Comparative analyses showed that despite being closely related, there may be significant variation among the venoms of freshwater stingrays, highlighting the importance of considering elicit care in handling different envenomation cases. Since hyaluronidase represents a major component of fish venom, we have performed phylogenetic and selective pressure analyses of this gene/protein across all fish with the available information. Results indicated an independent recruitment of the hyaluronidase into the stingray venom relative to that of venomous bony fish. The hyaluronidase residues were found to be mostly under negative selection, but 18 sites showed evidence of diversifying positive selection (P < 0.05). Our data provides new insight into stingray venom variation, composition, and selective pressure in hyaluronidase.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 19