Purification and characterisation of adenosine nucleosidase from Coffea arabica young leaves

• Tipo: Artigo em Revista Científica Internacional
• Data: 2005

Autores

Alexandre Campos (Autor) Outra; Rijo Johansen, MJ (Autor) Outra; Carneiro F (Autor) FMUP; Fevereiro, P (Autor) Outra

Revista

Título: Phytochemistry

Vol. 66

Páginas: 147-151

ISSN: 0031-9422

Editora: Elsevier

Indexação

ISI Web of Knowledge - 11 Citações

Scopus - 12 Citações

Outras Informações

ID Authenticus: P-000-6FQ

DOI: 10.1016/j.phytochem.2004.11.018

Abstract (EN): An adenosine nucleosidase (ANase) (EC 3.2.2.7) was purified from young leaves of Coffea arabica L. cv. Catimor. A sequence of fractionating steps was used starting with ammonium sulphate salting-out, followed by anion exchange, hydrophobic interaction and gel filtration chromatography. The enzyme was purified 5804-fold and a specific activity of 8333 nkatmg(-1) protein was measured. The native enzyme is a homodimer with an apparent molecular weight of 72 kDa estimated by gel filtration and each monomer has a molecular weight of 34.6 kDa, estimated by SDS-PAGE. The enzyme showed maximum activity at pH 6.0 in citrate-phosphate buffer (50 mM). The calculated K-m is 6.3 muM and V-max 9.8 nKat.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 5