Laboratory Research - cardiovascular molecular evaluation.

Code:

OPT19

Acronym:

ILAMC14

Keywords
Classification	Keyword
OFICIAL	Medicine

Instance: 2020/2021 - 1S (of 14-09-2020 to 07-02-2021)

Active?	Yes
Responsible unit:	Surgery and Physiology Department
Course/CS Responsible:	Integrated Master in Medicine

Cycles of Study/Courses

Acronym	No. of Students	Study Plan	Curricular Years	Credits UCN	Credits ECTS	Contact hours	Total Time
MIMED	9	Mestrado Integrado em Medicina- Plano oficial 2013 (Reforma Curricular)	2	-	3	28	81
			3

Teaching language

Suitable for English-speaking students

Objectives

Students are expected to be able to acquire basic knowledge and skills which will enable them to participate in scientific projects on cardiovascular sciences requiring laboratory techniques of molecular biology and histologic and morphologic evaluation.

Learning outcomes and competences

• Learn laboratory safety and good practices applied to the learned techniques.


• Master at least two research techniques of molecular biology and histology with application in cardiovascular research, such as fibroblast cell culture, neonatal organotypic cultures, extraction and quantification of proteins, extraction and quantification of nucleic acids, histology samples handling, ELISA or HPLC.


• Design of an experimental protocol using at least two molecular biology or histology techniques.


• Solve scientific problems associated with the used techniques.


• Critical assess scientific papers related to the learnt techniques.


• Analyze and present scientific results obtained in the course of this curricular unit.

Working method

Presencial

Program

1. Good practice and lab safety.

2. Design of an experimental protocol.

3. Tissue studies: morphometric analysis, immunohistochemestry and microscopy.

4. DNA, mRNA and proteins extraction from biologic products.

5. Transcriptome analysis: mRNA quantification.

6. Genome analysis: DNA quantification.

7. Proteomic analysis: protein quantification.

8. Data analysis and presentation.

Mandatory literature

Falcao-Pires, I., Gavina, C., & F Leite-Moreira, A. ; Understanding the Molecular and Cellular Changes Behind Aortic Valve Stenosis. , Current pharmaceutical biotechnology, 13(13), 2012
Francisco Amado, Pedro domingues, Maria do rosário Domingues, Rita Ferreira, Rui Vitorino; Análise de Proteínas - Guia de Laboratório, 100Luz, 2013. ISBN: 978-989-8448-23-1

Comments from the literature

Bibliography will include accompanying texts and scientific papers with relevance to the topics under study.

Teaching methods and learning activities

 

                Practice in the lab: 14 hours

                Tutorials: 12 hours

                Evaluation: 2 hours

Evaluation Type

Distributed evaluation without final exam

Assessment Components

Designation	Weight (%)
Participação presencial	50,00
Apresentação/discussão de um trabalho científico	50,00
Total:	100,00

Amount of time allocated to each course unit

Designation	Time (hours)
Apresentação/discussão de um trabalho científico	1,00
Estudo autónomo	24,00
Frequência das aulas	28,00
Trabalho de investigação	20,00
Trabalho laboratorial	8,00
Total:	81,00

Eligibility for exams

According to the current pedagogical regulation of FMUP.

Calculation formula of final grade

Students will be assessed based on their contact/interaction with professors (50% of the final grade) divided as follows:

Attendance (10%)

Participation, initiative and scientific interest (40%)

 

Oral presentation: 15-min presentation based on the work carried out and/or a related theme previously established (50% of the final grade).