Purification and characterisation of adenosine nucleosidase from Coffea arabica young leaves

• Type: Article in International Scientific Journal
• Date: 2005

Authors

Alexandre Campos (Author) Other; Rijo Johansen, MJ (Author) Other; Carneiro F (Author) FMUP; Fevereiro, P (Author) Other

Journal

Title: Phytochemistry

Vol. 66

Pages: 147-151

ISSN: 0031-9422

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 11 Citations

Scopus - 12 Citations

Other information

Authenticus ID: P-000-6FQ

DOI: 10.1016/j.phytochem.2004.11.018

Abstract (EN): An adenosine nucleosidase (ANase) (EC 3.2.2.7) was purified from young leaves of Coffea arabica L. cv. Catimor. A sequence of fractionating steps was used starting with ammonium sulphate salting-out, followed by anion exchange, hydrophobic interaction and gel filtration chromatography. The enzyme was purified 5804-fold and a specific activity of 8333 nkatmg(-1) protein was measured. The native enzyme is a homodimer with an apparent molecular weight of 72 kDa estimated by gel filtration and each monomer has a molecular weight of 34.6 kDa, estimated by SDS-PAGE. The enzyme showed maximum activity at pH 6.0 in citrate-phosphate buffer (50 mM). The calculated K-m is 6.3 muM and V-max 9.8 nKat.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 5