Simultaneous determination of flavonoids, phenolic acids, and coumarins in seven medicinal species by HPLC/diode-array detector

• Tipo: Artigo em Revista Científica Internacional
• Data: 1998

Autores

Andrade, PB (Autor) FFUP; Seabra, RM (Autor) Outra; Valentao, P (Autor) Outra; Areias, F (Autor) Outra

Revista

Título: Journal of Liquid Chromatography and Related Technologies

Vol. 21

Páginas: 2813-2820

ISSN: 1082-6076

Editora: Taylor & Francis

Indexação

ISI Web of Knowledge - 37 Citações

Scopus - 38 Citações

Classificação Científica

FOS: Ciências exactas e naturais > Química

Outras Informações

ID Authenticus: P-001-9BY

DOI: 10.1080/10826079808003444

Abstract (EN): A simple and accurate reversed phase HPLC procedure is proposed for the determination of 19 phenolic compounds (flavonoids, phenolic acids, and coumarins) in seven medicinal species. The sample preparation involved extraction, alkaline and acid hydrolysis, and purification through a Bond-Elut C18 column. The chromatographic separation was achieved using a reversed phase column Spherisorb ODS2 (5 mu m; 25.0 x 0.46 cm). Gradient elution was carried out using water-formic acid (19:1) (A) and methanol (B). A diode-array detector monitored the effluent and chromatograms were recorded at 280, 320, and 350 nm. The optimised methodology seems to be useful for the phytochemical analysis of plant extracts. A close correlation between the phenolic compound patterns and the botanical origin of plants was found.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 8