Simultaneous determination of flavonoids, phenolic acids, and coumarins in seven medicinal species by HPLC/diode-array detector

• Type: Article in International Scientific Journal
• Date: 1998

Authors

Andrade, PB (Author) FFUP; Seabra, RM (Author) Other; Valentao, P (Author) Other; Areias, F (Author) Other

Journal

Title: Journal of Liquid Chromatography and Related Technologies

Vol. 21

Pages: 2813-2820

ISSN: 1082-6076

Publisher: Taylor & Francis

Indexing

ISI Web of Knowledge - 37 Citations

Scopus - 38 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-001-9BY

DOI: 10.1080/10826079808003444

Abstract (EN): A simple and accurate reversed phase HPLC procedure is proposed for the determination of 19 phenolic compounds (flavonoids, phenolic acids, and coumarins) in seven medicinal species. The sample preparation involved extraction, alkaline and acid hydrolysis, and purification through a Bond-Elut C18 column. The chromatographic separation was achieved using a reversed phase column Spherisorb ODS2 (5 mu m; 25.0 x 0.46 cm). Gradient elution was carried out using water-formic acid (19:1) (A) and methanol (B). A diode-array detector monitored the effluent and chromatograms were recorded at 280, 320, and 350 nm. The optimised methodology seems to be useful for the phytochemical analysis of plant extracts. A close correlation between the phenolic compound patterns and the botanical origin of plants was found.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8