Proteolysis activation and proteome alterations in murine skeletal muscle submitted to 1 week of hindlimb suspension

• Tipo: Artigo em Revista Científica Internacional
• Data: 2009

Autores

ferreira, r (Autor) Outra; vitorino, r (Autor) Outra; neuparth, mj (Autor) Outra; appell, hj (Autor) Outra; duarte, ja (Autor) FADEUP; amado, f (Autor) Outra

Revista

Título: European Journal of Applied Physiology

Vol. 107

Páginas: 553-563

ISSN: 1439-6319

Editora: Springer Nature

Indexação

ISI Web of Knowledge - 22 Citações

Scopus - 27 Citações

Classificação Científica

FOS: Ciências médicas e da saúde > Medicina básica

Outras Informações

ID Authenticus: P-003-EKN

DOI: 10.1007/s00421-009-1151-1

Abstract (EN): The aim of this study was to investigate the temporal involvement of different proteolytic systems and muscle proteome changes during experimental disuse atrophy (up to 1 week hindlimb suspension, HS) in murine gastrocnemius muscle. The results showed that proteolysis, cytoprotection mechanisms and signs of cellular infiltration occurred very early. After 1 day of HS, signals of lysosomal activation, rather than programmed cell death (apoptosis), seem to trigger protein breakdown in the whole skeletal muscle. Moreover, the ubiquitin-proteasome pathway remained elevated later whereas all other proteolytic parameters returned to control values when atrophy was fully established. Using proteomics, evidence is provided for metabolic alterations toward glycolysis and for cytoskeleton remodelling suggestive of reduced capacity for force generation. Overall, our data highlight an early and coordinated time-dependent activation of proteolysis, which explains the global proteome alterations observed in gastrocnemius under atrophic conditions.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 11