Laccase production by immobilized mycelia of Peniophora cinerea and Trametes versicolor

• Tipo: Artigo em Revista Científica Internacional
• Data: 2013

Autores

Sara C. Silvério (Autor) FEUP; Sérgio Moreira (Autor) Outra; Adriane M. F. Milagres (Autor) Outra; Eugénia A. Macedo (Autor) FEUP; José A. Teixeira (Autor) Outra; Solange I. Mussatto (Autor) Outra

Revista

Título: Bioprocess and Biosystems Engineering

Vol. 36 Nº 3

Páginas: 365-373

ISSN: 1615-7591

Editora: Springer Nature

Indexação

ISI Web of Knowledge

ISI Web of Science

Scopus

Classificação Científica

FOS: Ciências da engenharia e tecnologias

CORDIS: Ciências Tecnológicas

Projetos Associados

LA 20 - PROJECTO ESTRATÉGICO - LA 20 - 2013-2014

Outras Informações

DOI: 10.1007/s00449-012-0793-2

Abstract (EN): The production of laccase by immobilized mycelia of Peniophora cinerea and Trametes versicolor was studied. In an initial stage, experimental assays were performed in Erlenmeyer flasks using free and immobilized mycelium, and the performance of the fungal strains to produce the enzyme was compared. Both fungi adhered into the support material (a synthetic fiber), growing not only on the surface but also in the interspaces of the fibers. Immobilization of P. cinerea provided a 35-fold increase in laccase production when compared to the production obtained by using free mycelium. On the other hand, immobilization of T. versicolor caused a decrease in laccase activity. A comparison between the strains revealed that immobilized P. cinerea (3,500 U/L) surpassed the enzyme production by free T. versicolor (800 U/L). When the conditions that gave the best laccase production to each fungus were employed in a stirred tank bioreactor, very low laccase production was observed for both the cases, suggesting that shear stress and mycelia damage caused by the agitation impellers negatively affected the enzyme production. © 2012 Springer-Verlag.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 9