The use of Escherichia coli total antigens as a complementary approach to address seropositivity to Leishmania antigens in canine leishmaniosis

• Tipo: Artigo em Revista Científica Internacional
• Data: 2017

Autores

Lima, C (Autor) Outra; Mesquita, JR (Autor) Outra; Brancal, H (Autor) Outra; Vahlenkamp, T (Autor) Outra; Teixeira, AR (Autor) Outra; Cardoso, L (Autor) Outra; Amorim, CG (Autor) FFUP; Santarem, N (Autor) Outra; Anabela Cordeiro da Silva (Autor) FFUP

Revista

Título: Parasitology

Vol. 144

Páginas: 1384-1393

ISSN: 0031-1820

Editora: Cambridge University Press

Indexação

ISI Web of Knowledge - 5 Citações

Scopus - 5 Citações

Outras Informações

ID Authenticus: P-00M-XBP

DOI: 10.1017/s0031182017000713

Abstract (EN): Canine leishmaniosis (CanL) is a major veterinary concern and a public health issue. Serological data are essential for disease management. Several antigens used in serological assays have specificity related problems preventing relevant seropositivity values establishment. Herein we report significant seropositivity level disparity in a study cohort with 384 dogs from eight countries, for antigens traditionally used in CanL - soluble promastigote Leishmania antigens (SPLA) and K39 recombinant protein (rK39): 43.8 and 2.9% for SPLA and rK39, respectively. To better understand the reasons for this disparity, CanL-associated serological response was characterized using, for complement serological evaluation, a ubiquitous antigen - soluble Escherichia coli antigens (SECAs). Using cohorts of CanL dogs and dogs without clinical evidences of CanL from non-endemic regions of Portugal, the serological response of CanL animals followed specific trend of seropositivity rK39 > SPLA > SECA absent in non-diseased animals. Using receiver operating characteristic curve analysis, these characteristic trends were converted in ratios, SPLA/SECA, rK39/SECA and rK39/SPLA, that presented high predictive for discriminating the CanL cohort that was potentiated when applied in a scoring system involving positivity to four out of five predictors (rK39, SPLA, SPLA/SECA, rK39/SECA and rK39/SPLA). In fact, this approach discriminated CanL with similar sensitivity/specificity as reference antigens, diminishing seropositivity in European cohort to 1.8%. Ultimately, non-related antigens like SECA and seropositivity ratios between antigens enable different perspectives into serological data focusing on the search of characteristic serological signatures and not simple absolute serology values contributing to comprehensive serological status characterization.

Idioma: Inglês

Tipo (Avaliação Docente): Científica

Nº de páginas: 10