Resumo (PT):
Abstract (EN):
In this work, a highly sensitive label-free immunosensor for detection of the main biomarker of
Alzheimer’s disease (AD), amyloid beta 1–42 (A (1–42)), is presented. A gold electrode was modified
with a mercaptopropionic acid (MPA) self-assembled monolayer, electrodeposited gold nanoparticles
(AuNPs) and a monoclonal antibody mAb DE2B4 to recognize A; all the relevant experimental variables
were optimized. Antibodies were functionalized through chemical modification (thiolation) to promote
the antibody immobilization on the AuNPs surface with proper orientation which enabled the direct
detection of A(1–42). Scanning electron microscopy, square-wave voltammetry and electrochemical
impedance spectroscopy were used to characterize the construction of the biosensor. Using the proposed immunosensor, A(1–42) was specifically detected within the linear range of 10–1000 pg mL−1
with a 5.2 pg mL−1 and 17.4 pg mL−1 detection and quantification limit, respectively; recovery values for
the tested spiking levels ranged from 90.3 to 93.6%. The immunosensor enables rapid, accurate, precise,
reproducible and highly sensitive detection (14.6%reduction mL pg−1) of A with low-cost and opens the
possibilities for diagnostic ex vivo applications and research-based in vivo studies.
Idioma:
Inglês
Tipo (Avaliação Docente):
Científica
Nº de páginas:
9