Laboratory Research ¿ In vitro functional evaluation
Keywords |
Classification |
Keyword |
OFICIAL |
Medicine |
Instance: 2017/2018 - 1S
Cycles of Study/Courses
Teaching language
Suitable for English-speaking students
Objectives
Students are expect to acquire basic knowledge and skills which will enable them to participate in scientific projects requiring laboratory techniques for in vitro functional assessment of myocardium, blood vessels and smooth and skeletal muscle.
Learning outcomes and competences
- Learn laboratory safety and good practices applied to the learned techniques.
- Master at least two in vitro research techniques of functional assessment, such as, functional assessment of intact and skinned cardiomyocytes, vascular function assessment, myocardial multicellular preparations functional assessment, Langendorff or working heart preparations.
- Design of an experimental protocol using at least two functional assessment techniques.
- Solve scientific problems associated to the used techniques.
- Critical assess scientific papers related to the learnt techniques.
- Analyze and present scientific results obtained in the course of this curricular unit.
Working method
Presencial
Program
1.Good practice and lab safety
2.Design of an experimental protocol
3.In vitro functional studies
a)Cell culture
b)Functional evaluation of isolated cardiomyocytes
c)Functional evaluation of multicellular cardiac preparations
d)Functional evaluation of blood vessels
e)Functional evaluation of smooth muscle
f)Functional evaluation of skeletal muscle
g)Langendorff preparation
4.Data analysis and presentation
Mandatory literature
Falcão- Pires, I., Leite-Moreira, AF. ; In vitro Experimental Assessment of Cardiac Function In D.V. Cokkinos (Ed.), Introduction to Translational Cardiovascular Research., Research. Switzerland: Springer International Publishing, 2014
Comments from the literature
Bibliography will include accompanying texts and scientific papers with relevance to the topics under study.
Teaching methods and learning activities
Practice in the lab: 14 hours
Tutorials: 12 hours
Evaluation: 2 hours
Evaluation Type
Distributed evaluation without final exam
Assessment Components
Designation |
Weight (%) |
Participação presencial |
75,00 |
Prova oral |
25,00 |
Total: |
100,00 |
Calculation formula of final grade
Students will be assessed based on their contact/interaction with professors (75% of the final grade) divided as follows:
Attendance (20%)
Participation and scientific interest (20%)
Commitment and initiative (20%)
Understanding of the methodology (15%)
and on an oral presentation: 15-min presentation based on the work carried out and/or a related theme previously established (25% of the final grade).