Laboratory Investigation

Code:

MI010

Acronym:

MI010

Keywords
Classification	Keyword
OFICIAL	Medicine

Instance: 2014/2015 - SP

Active?	Yes
Responsible unit:	Physiology and Cardiothoracic Surgery Department
Course/CS Responsible:	Integrated Master in Medicine

Cycles of Study/Courses

Acronym	No. of Students	Study Plan	Curricular Years	Credits UCN	Credits ECTS	Contact hours	Total Time
MIMED	2	Mestrado Integrado em Medicina 2007	6	-	3	32	81
		Mestrado Integrado em Medicina- Plano oficial 2013 (Reforma Curricular)	6	-	3	32	81

Teaching language

Portuguese

Objectives

OBJECTIVES

To provide the student basic knowledge and practical skills for working in scientific projects requiring laboratory techniques for functional, morphologic and molecular biology assessment.


COMPETENCES

1. To design an experimental protocol.

2. Critical thinking and problem solving capacities.

3. To critically evaluate a scientific paper.

4. To learn at least two different laboratory techniques.

5. To learn how to analyze and present scientific results.

Learning outcomes and competences

Resultados de aprendizagem e competências

Working method

Presencial

Program

Design of an experimental protocol; Laboratory security; Animal models of disease: surgical; pharmacologic; knockouts and transgenics; Study of organs and systems: functional and dynamic models; Study of tissues; morphometry and immunocytochemistry; Microscopy; Study of the cell: cell culture; DNA, mRNA and protein isolation from biologic samples; Transcriptom analysis; mRNA quantification; Genomics analysis; DNA quantification; Proteome analysis; Protein quantification. Analysis, interpretation and presentation of results.

Mandatory literature

n/a; n/a

Teaching methods and learning activities

Total: 32 hours

Tutorials: 16 hours
Laboratorial practice: 16 hours
Total of working hours: 81 hours

keywords

Health sciences > Medical sciences > Medicine > Physiology
Natural sciences > Biological sciences > Laboratory animal science

Evaluation Type

Distributed evaluation with final exam

Assessment Components

Designation	Weight (%)
Participação presencial	50,00
Trabalho laboratorial	50,00
Total:	100,00

Eligibility for exams

Continuous Assessment done by the teaching staff during contact hours.

Calculation formula of final grade

1. Continuous Assessment done by the teaching staff during contact hours, weighting 75% of the final classification.
2. An oral communication limited to 15 minutes where the student has to present the results of the developed laboratory work. Represents 25% of the final classification.