Physical exercise prevents and mitigates non-alcoholic steatohepatitis-induced liver mitochondrial structural and bioenergetics impairments

• Type: Article in International Scientific Journal
• Date: 2014

Authors

goncalves, io (Author) Other; passos, e (Author) Other; rocha-rodrigues, s (Author) Other; diogo, cv (Author) Other; torrella, jr (Author) Other; rizo, d (Author) Other; viscor, g (Author) Other; santos-alves, e (Author) Other; marques-aleixo, i (Author) Other; oliveira, pj (Author) Other; ascensao, aamr (Author) Other; magalhaes, jf (Author) FADEUP

Journal

Title: Mitochondrion

Vol. 15

Pages: 40-51

ISSN: 1567-7249

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 15 Citations

Scopus - 4 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-009-DRX

DOI: 10.1016/j.mito.2014.03.012

Abstract (EN): Exercise is considered a non-pharmacological tool against several lifestyle disorders in which mitochondrial dysfunction is involved. The present study aimed to analyze the preventive (voluntary physical activity-VPA) and therapeutic (endurance training-ET) role of exercise against non-alcoholic steatohepatitis (NASH)-induced liver mitochondrial dysfunction. Sixty male Sprague-Dawley rats were divided into standard-diet sedentary (SS, n = 20), standard-diet VPA (SVPA, n = 10), high-fat diet sedentary (HS, n = 20) and high-fat diet VPA (HVPA, n = 10). After 9 weeks of diet-treatment, half of SS and HS animals were engaged in an ET program (SET and HET) for 8 weeks, 5 days/week and 60 min/day. Liver mitochondrial oxygen consumption and transmembrane-electric potential (Delta Psi) were evaluated in the presence of glutamate-malate (G/M), palmitoylmalate (P/M) and succinate (S/R). Mitochondrial enzymes activity, lipid and protein oxidation, oxidative phosphorylation (OXPHOS) subunits, cytochrome c, adenine nucleotide translocator (ANT) and uncoupling protein-2 (UCP2) content were assessed. HS groups show the histological features of NASH in parallel with decreased AT and respiratory control (RCR) and ADP/O ratios (G/M and P/M). A state 3 decrease (G/M and S/R), FCCP-induced uncoupling respiration (S/R) and ANT content were also observed. Both exercise types counteracted oxygen consumption (RCR, ADP/O and FCCP-uncoupling state) impairments and improved Delta Psi (lag-phase). In conclusion, exercise prevented or reverted (VPA and ET, respectively) the bioenergetic impairment induced by NASH, but only ET positively remodeled NASH-induced liver structural damage and abnormal mitochondria. It is possible that alterations in inner membrane integrity and fatty acid oxidation may be related to the observed phenotypes induced by exercise.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 12