Microsatellite instability, mitochondrial DNA large deletions, and mitochondrial DNA mutations in gastric carcinoma

• Type: Article in International Scientific Journal
• Date: 2001

Authors

Maximo, V (Author) Other; Soares, P (Author) FMUP; Seruca, R (Author) FMUP; Rocha, AS (Author) Other; Castro, P (Author) Other; Sobrinho Simoes, M (Author) FMUP

Journal

Title: Genes Chromosomes and Cancer

Vol. 32

Pages: 136-143

ISSN: 1045-2257

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 103 Citations

Scopus - 107 Citations

Scientific classification

FOS: Medical and Health sciences > Clinical medicine

Other information

Authenticus ID: P-000-T4A

DOI: 10.1002/gcc.1175

Abstract (EN): Mitochondrial DNA (mtDNA) large deletions and mtDNA mutations have been demonstrated in various types of human cancer. The relationship between the occurrence of such alterations and the nuclear microsatellite instability (MSI) status of the neoplastic cells remains controversial. In an attempt to clarify the situation in gastric carcinoma, we studied, by PCR/SSCP and sequencing, five mitochondrial genes and two D-loop regions In 32 gastric carcinomas that had been previously screened for MSI and mitochondrial common deletion. MtDNA alterations were detected in 26 carcinomas (81%). All the mtDNA mutations, which occurred mainly In the D-loop and ND1 and ND5 genes, were transitions. D-loop alterations (insertions and/or deletions) were not significantly associated with mutations In the coding regions. There was a trend towards an inverse relationship between the occurrence of mitochondrial common deletion and mtDNA mutations. No significant relationship was observed between MSI status and mtDNA mutations, whereas the mitochondrial common deletion appeared to be almost exclusively restricted to MSI-negative tumors. The latter finding-almost no gastric carcinoma with MSI-positive phenotype has Targe deletions of mtDNA-needs to be confirmed in a larger series and in tumors from other organs. (C) 2001 Wiley-Liss, Inc.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8