Endurance training and chronic intermittent hypoxia modulate in vitro salicylate-induced hepatic mitochondrial dysfunction

• Type: Article in International Scientific Journal
• Date: 2012

Authors

ascensao, a (Author) FADEUP; goncalves, io (Author) Other; lumini-oliveira, j (Author) Other; marques-aleixo, i (Author) Other; dos passos, e (Author) Other; rocha-rodrigues, s (Author) Other; machado, ng (Author) Other; moreira, ac (Author) Other; oliveira, pj (Author) Other; torrella, jr (Author) Other; magalhaes, j (Author) FADEUP

Journal

Title: Mitochondrion

Vol. 12

Pages: 607-616

ISSN: 1567-7249

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 12 Citations

Scopus - 12 Citations

Scientific classification

FOS: Natural sciences > Biological sciences

Other information

Authenticus ID: P-002-3R1

DOI: 10.1016/j.mito.2012.10.007

Abstract (EN): Mitochondrial function is modulated by multiple approaches including physical activity, which can afford cross-tolerance against a variety of insults. We therefore aimed to analyze the effects of endurance-training (ET) and chronic-intermittent hypobaric-hypoxia (IHH) on liver mitochondrial bioenergetics and whether these effects translate into benefits against in vitro salicylate mitochondrial toxicity. Twenty-eight young-adult male rats were divided into normoxic-sedentary (NS), normoxic-exercised (NE), hypoxic-sedentary (HS) and hypoxic-exercised (HE). ET consisted of 1 h/days of treadmill running and IHH of simulated atmospheric pressure of 49.3 kPa 5 h/days during 5 weeks. Liver mitochondrial oxygen consumption, transmembrane-electric potential (Delta Psi) and permeability transition pore induction (MPTP) were evaluated in the presence and absence of salicylate. Aconitase, MnSOD, caspase-3 and 8 activities, - SH, MDA, SIRT3, Cyp D, HSP70, and OXPHOS subunit contents were assessed. ET and IHH decreased basal mitochondrial state-3 and state-4 respiration, although no alterations were observed in Delta Psi, endpoints evaluated in control mitochondria. In the presence of salicylate, ET and IHH decreased state-4 and lag-phase of ADP-phosphoiylation. Moreover, ADP-lag phase in hypoxic was further lower than in normoxic groups. Neither ET nor IHH altered the susceptibility to calcium-induced MPTP. IHH lowered MnSOD and increased aconitase activities. ET and IHH decreased caspase 8 activity whereas no effect was observed on caspase 3. The levels of SIRT3 increased with ET and IHH and Cyp D decreased with IHH. Data suggest that ET and IHH do not alter general basal liver mitochondrial function, but may attenuate some adverse effects of salicylate.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10