Uptake of [H-3]-adrenaline by freshly isolated rat hepatocytes: putative involvement of P-glycoprotein

• Type: Article in International Scientific Journal
• Date: 1998

Authors

Martel, F (Author) FMUP; Calhau, C (Author) FCNAUP; Martins, MJ (Author) FMUP; Azevedo, I (Author) Other

Journal

Title: Journal of Autonomic Pharmacology

Vol. 18

Pages: 57-64

ISSN: 0144-1795

Publisher: Blackwell

Indexing

ISI Web of Knowledge - 14 Citations

Scopus - 14 Citations

Scientific classification

FOS: Medical and Health sciences > Basic medicine

Other information

Authenticus ID: P-001-8K9

DOI: 10.1046/j.1365-2680.1998.1810057.x

Abstract (EN): 1 The liver has an important role in the elimination of circulating catecholamines. Adrenaline and noradrenaline are avidly taken up and metabolized by rat hepatocytes, but the nature of the mechanism(s) involved remains partially unknown. 2 The aim of this work was to further characterize the uptake of catecholamines by isolated rat hepatocytes. For that purpose, the effects of a series of chemically unrelated compounds, including substrates/inhibitors of P-glycoprotein, on [H-3]-adrenaline removal was investigated. 3 Freshly isolated rat hepatocytes were incubated in Krebs-Henseleit solution at 37 degrees C with 50 nM [H-3]-adrenaline for 5 min. Removal of [H-3]-adrenaline was calculated as the sum of [H-3]-adrenaline present in cells, and its [H-3]-metabolites present both in cells and in the incubation medium. Radioactivity was determined by liquid scintillation counting. 4 Verapamil, quinidine, 1-methyl-4-phenylpyridinium, cimetidine, tetraethylammonium, d-tubocurarine, taurocholate, daunomycin and vinblastine (100 mu M), progesterone, bilirubin (200 mu M), vecuronium (45 mu M), and amiloride (1 mM) significantly reduced [H-3]-adrenaline removal. On the other hand, cyclosporine A (100 mu M) apparently had no effect. The O-methylated metabolite of adrenaline, metanephrine (30 mu M), produced a 40% reduction of [H-3]-adrenaline removal. 5 Vinblastine and corticosterone produced concentration-dependent decreases of [H-3]-adrenaline removal, with IC50 values of 23.3 and 116.0 mu M, respectively. 6 In the presence of verapamil (100 mu M), desipramine (1 mu M) was devoid of significant effect on [H-3]-adrenaline removal. Corticosterone (40 mu M) produced a further decrease (+/- 50%) on removal of the [H-3]-amine. 7 Removal of [H-3]-adrenaline by isolated cells did not show pH-dependence since an increase or a decrease in the pH of incubation medium (to 8.2 or 6.2, respectively) caused no alteration of that parameter. 8 In conclusion, [H-3]-adrenaline is efficiently removed and subsequently metabolized by isolated rat hepatocytes. The results are compatible with the involvement of multiple mechanisms in the hepatic uptake of this amine including the type I and the type II hepatic transporters for organic cations, uptake(2) and P-glycoprotein.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8