Chitosan conjugates for DNA delivery

• Type: Article in International Scientific Journal
• Date: 2013

Authors

Diana Paiva (Author) FEUP; Galya Ivanova (Author) Other; Maria do Carmo Pereira (Author) FEUP; Sandra Rocha (Author) FEUP

Journal

Title: Physical Chemistry Chemical Physics

Vol. 15 No. 28

Pages: 11893-11899

ISSN: 1463-9076

Publisher: Royal Society of Chemistry

Indexing

ISI Web of Knowledge - 9 Citations

ISI Web of Science

Scopus - 12 Citations

INSPEC

Scientific classification

FOS: Natural sciences > Chemical sciences

CORDIS: Technological sciences ; Health sciences

Associated Projects

Referência: PTDC/QUI-BIQ/102827/2008 - Concepção de péptidos fluorados para inibição da agregação do péptido beta-amilóide e avaliação da sua actividade biológica

PTDC/QUI-BIQ/118076/2010 - Liposomas funcionalizados com anticorpos para a doença de Alzheimer

Other information

Authenticus ID: P-006-G6J

DOI: 10.1039/c3cp51215k

Abstract (EN): Graft copolymers of chitosan and maltodextrin were synthesized by reductive amination of a low molecular weight chitosan. The degree of substitution is 70% or above, as quantified by nuclear magnetic resonance spectroscopy, at molar ratios of chitosan glucosamine units and maltodextrin of 1 : 1 and 1 : 5. The high substitution degree generates a water-soluble compound at pH 7.4 by reducing the amino groups of chitosan. In addition, the copolymer self-assembles into spherical nanoparticles with diameters of about 300 nm. The mass polymer/DNA ratios at the isoelectric point are within the range of 3-3.5 for chitosan-maltodextrin nanoparticles as determined by electrophoretic mobility measurements. These results confirm that DNA interacts efficiently with the copolymer nanoparticles indicating a potential application of the system for DNA delivery.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 7