The effect of a fluorinated cholesterol derivative on the stability and physical properties of cationic DNA vectors

• Type: Article in International Scientific Journal
• Date: 2013

Authors

Diana Paiva (Author) FEUP; Albertoo Martín-Molina (Author) Other; Isabel Cardoso (Author) Other; Manuel Quesada-Pérez (Author) Other; Maria do Carmo Pereira (Author) FEUP; Sandra Rocha (Author) FEUP

Journal

Title: Soft Matter

Vol. 9 No. 2

Pages: 401-409

ISSN: 1744-683X

Publisher: Royal Society of Chemistry

Indexing

ISI Web of Knowledge - 14 Citations

ISI Web of Science

Scopus - 14 Citations

Scientific classification

FOS: Engineering and technology > Materials engineering

CORDIS: Health sciences ; Technological sciences

Associated Projects

Referência: PTDC/QUI-BIQ/102827/2008 - Concepção de péptidos fluorados para inibição da agregação do péptido beta-amilóide e avaliação da sua actividade biológica

PTDC/QUI-BIQ/118076/2010 - Liposomas funcionalizados com anticorpos para a doença de Alzheimer

Other information

Authenticus ID: P-002-266

DOI: 10.1039/c2sm27236a

Abstract (EN): Liposomes of the cationic lipid DOTAP (1,2-dioleoyl-3-trimethylammonium-propane) and the fluorinated cholesterol derivative heptafluorocholesterol (F7-CHOL) were tested at the molar ratio of 1 : 1 for DNA compaction and transfection. Their properties were correlated with the characteristics of the well-known system, DOTAP and cholesterol (CHOL). The mass lipid-DNA (L/D) ratios at the isoelectric point were within the ranges 3-4 for DOTAP: CHOL and 4-5 for DOTAP: F7-CHOL, as determined by electrophoretic mobility measurements. These results and the ethidium bromide fluorescence intercalation assays confirmed that more DOTAP: F7-CHOL liposomes are needed to compact the same amount of DNA as DOTAP: CHOL. The phase diagrams of aggregation and re-entrant condensation phenomena obtained by phenomenological theory support this conclusion and also establish that the liposome-DNA binding is stronger in the case of the DOTAP:F7-CHOL-DNA system. The stability rates of both liposomes in the presence of DNA and their transfection efficiencies were similar, which support the application of heptafluorocholesterol as a helper lipid in cationic liposomes for DNA delivery.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 9