Simultaneous determination of E-2-nonenal and beta-damascenone in beer by reversed-phase liquid chromatography with UV detection

• Type: Article in International Scientific Journal
• Date: 2004

Authors

Guido, LF (Author) FCUP; Carneiro, JR (Author) Other; Santos, JR (Author) REIT; Almeida, PJ (Author) FCUP; Rodrigues, JA (Author) FCUP; Barros, AA (Author) FCUP

Journal

Title: Journal of Chromatography A

Vol. 1032

Pages: 17-22

ISSN: 0021-9673

Publisher: Elsevier

Indexing

ISI Web of Knowledge - 30 Citations

Scopus - 30 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-000-AVK

DOI: 10.1016/j.chroma.2003.12.026

Abstract (EN): A method for the simultaneous determination of E-2-nonenal and beta-damascenone in beer by reversed-phase liquid chromatography using UV detection is presented. The method consists of beer steam distillation, followed by an extraction/concentration step using Sep-Pak Plus C-18 RP cartridges and determination by HPLC at 226 nm UV-absorption maximum. The identity of the compounds was confirmed by GC analysis with MS detection of the isolated fractions. A recovery factor of approximately 80% was obtained for beta-damascenone with a R.S.D. of 3%. E-2-Nonenal and beta-damascenone were monitored in a comparative study of fresh and either naturally and forced aged beer. The results obtained show that both compounds have a similar behaviour through an extended storage of beer and consequently can be used as good analytical markers of beer ageing. Nevertheless, the use of beta-damascenone seems to be more convenient because this compound appears in beer in higher concentrations than E-2-nonenal, thus making it easier to measure.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 6