Samarium doped glass-reinforced hydroxyapatite with enhanced osteoblastic performance and antibacterial properties for bone tissue regeneration

• Type: Article in International Scientific Journal
• Date: 2014

Authors

Diana Santos Morais (Author) Other; Joao Coelho (Author) Other; Ferraz, MP (Author) Other; Gomes PS (Author) FMDUP; Fernandes MH (Author) FMDUP; Nandyala Sooraj Hussain (Author) Other; Jose Domingos Santos (Author) FEUP; Lopes, MA (Author) FEUP

Journal

Title: Journal of Materials Chemistry B

Vol. 2

Pages: 5872-5881

ISSN: 2050-7518

Publisher: Royal Society of Chemistry

Indexing

ISI Web of Knowledge - 45 Citations

ISI Web of Science

Scopus - 49 Citations

Scientific classification

FOS: Engineering and technology > Materials engineering

CORDIS: Technological sciences > Engineering > Biomaterial engineering

Other information

Authenticus ID: P-009-R40

DOI: 10.1039/c4tb00484a

Abstract (EN): A novel bioactive bone substitute with improved osteoblastic performance and effective antibacterial activity was developed, using a completely new approach based on samarium (Sm3+) doped P2O5 glass-reinforced hydroxyapatite composites (GR-HA). The composites were prepared by adding 2.5% (w/w) of the P2O5 glass to 97.5% (w/w) of HA. Four composites were developed, Le. one non-doped composite, and three Sm3+ doped composites prepared with the P2O5 glass containing 0.5, 1 and 2 (mol%) of Sm2O3. The composites were labeled as GR-HA_control, GR-HA_0.5Sm, GR-HA_1Sm and GR-HA_2Sm. The composites were physicochemical and mechanically characterized, namely performing SEM, EDS and XRD analysis and flexural bending strength (FBS) assessment. The incorporation of Sm3+ in the GR-HA matrix resulted in the presence of a residual Sm3+ containing phase besides HA, beta-TCP and alpha-TCP phases, increased surface hydrophilicity and slightly higher FBS. Sm3+ doped composites exhibited improved osteoblastic cell response, as evidenced by a better F-actin cytoskeleton organization and higher cell proliferation and expression of relevant osteoblastic genes. In addition, adhesion of Staphylococcus aureus and Staphylococcus epidermidis was greatly reduced on these composites. The improved osteoblastic behavior and the antibacterial effects were dependent on the amount of Samarium in the composite, this being particularly evident in the composite with a higher Sm3+ content. Therefore, the developed composite GR-HA_2Sm appears as a successful bone substitute with osteoconductive and antibacterial properties.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 10