Simultaneous determination of retinol, beta-carotene and alpha-tocopherol in adipose tissue by high-performance liquid chromatography

• Type: Article in International Scientific Journal
• Date: 2001

Authors

Casal, S (Author) FFUP; Macedo, B (Author) Other; Oliveira, MBPP (Author) FFUP

Journal

Title: Journal of Chromatography - Biomedical Applications

Vol. 763

Pages: 1-8

ISSN: 0378-4347

Publisher: Elsevier B.V.

Indexing

ISI Web of Knowledge - 38 Citations

Scopus - 41 Citations

Scientific classification

FOS: Natural sciences > Chemical sciences

Other information

Authenticus ID: P-000-SSD

DOI: 10.1016/s0378-4347(01)00349-8

Abstract (EN): A method is described for evaluation of fat-soluble vitamin in human adipose tissue with the aim to obtain, accurately and within the shortest analysis time, a time-integrated measure of exposure to vitamins from the diet. Fat tissue was deproteinized with ethanol and extracted with n-hexane. Normal-phase HPLC was performed in a Lichrosorb Si60 column with a gradient of n-hexane-2-propanol at 1 ml/min. Detection was accomplished using a diode-array system (for retinol and beta -carotene) in series with a fluorescence detector (alpha -tocopherol). The method was validated and applied to human adipose tissue in a total of 140 subjects. The mean contents found were 0.43, 0.84, 240.3 mug/g for retinol, beta -carotene and alpha -tocopherol, respectively. The method is sensitive enough for detecting the compounds in 1.6 mg of adipose tissue considering the lowest concentration found.

Language: English

Type (Professor's evaluation): Scientific

No. of pages: 8