HPLC-PAD-atmospheric pressure chemical ionization-MS metabolite profiling of cytotoxic carotenoids from the echinoderm Marthasterias glacialis (spiny sea-star)

• Type: Article in International Scientific Journal
• Date: 2010

Authors

Federico Ferreres (Author) Other; David M. Pereira (Author) FFUP; Angel Gil-Izquierdo (Author) Other; Patrícia Valentão (Author) FFUP; João Botelho (Author) Other; Teresa Mouga (Author) Other; Paula B. Andrade (Author) FFUP

Journal

Title: Journal of Separation Science

Vol. 33 No. 15

Pages: 2250-2257

ISSN: 1615-9306

Publisher: Wiley-Blackwell

Indexing

ISI Web of Knowledge - 13 Citations

Scopus - 14 Citations

Scientific classification

FOS: Medical and Health sciences > Other medical sciences

CORDIS: Health sciences > Pharmacological sciences > Pharmacognosy

Other information

Authenticus ID: P-003-4FT

Resumo (PT): An HPLC-PAD-atmospheric pressure chemical ionization-MS metabolite profiling analysis was conducted on the marine echinoderm Marthasterias glacialis (spiny sea-star). Bio-guided purification of the methanolic extract led to the isolation of several carotenoids, namely zeaxanthin, astaxanthin and lutein. These compounds were characterized using both UV–Vis characteristics and MS spectra interpretation. No previous works addressed the MS analysis of carotenoids present in this organism. The purified carotenoid fraction displayed a strong cell proliferation inhibition against rat basophilic leukemia RBL-2H3 (IC25=268 μg/mL) cancer cell line. Against healthy V79 (rat lung fibroblasts (IC25=411 μg/mL)) cell line, however, toxicity was lower, as it is desired for anti-cancer molecules. This study suggests that M. glacialis may constitute a good source of bioactive compounds that can be used as lead compounds for the pharmaceutical industry. <br> <br> Keywords:Carotenoids;Cytotoxic;HPLC-PAD-atmospheric pressure chemical ionization-MS;Marthasterias glacialis <br> <a target="_blank" href="http://onlinelibrary.wiley.com/doi/10.1002/jssc.201000197/abstract"> Texto integral</a> <br> <br>

Abstract (EN): An HPLC-PAD-atmospheric pressure chemical ionization-MS metabolite profiling analysis was conducted on the marine echinoderm Marthasterias glacialis (spiny sea-star). Bio-guided purification of the methanolic extract led to the isolation of several carotenoids, namely zeaxanthin, astaxanthin and lutein. These compounds were characterized using both UV–Vis characteristics and MS spectra interpretation. No previous works addressed the MS analysis of carotenoids present in this organism. The purified carotenoid fraction displayed a strong cell proliferation inhibition against rat basophilic leukemia RBL-2H3 (IC25=268 μg/mL) cancer cell line. Against healthy V79 (rat lung fibroblasts (IC25=411 μg/mL)) cell line, however, toxicity was lower, as it is desired for anti-cancer molecules. This study suggests that M. glacialis may constitute a good source of bioactive compounds that can be used as lead compounds for the pharmaceutical industry. <br> <br> Keywords:Carotenoids;Cytotoxic;HPLC-PAD-atmospheric pressure chemical ionization-MS;Marthasterias glacialis <br> <a target="_blank" href="http://onlinelibrary.wiley.com/doi/10.1002/jssc.201000197/abstract"> Full text </a> <br> <br>

Language: English

Type (Professor's evaluation): Scientific